OSA's Digital Library

Journal of Optical Technology

Journal of Optical Technology

| SIMULTANEOUS RUSSIAN-ENGLISH PUBLICATION

  • Vol. 78, Iss. 11 — Nov. 1, 2011
  • pp: 730–734

Using the method of depth reconstruction from focusing for microscope images

A. N. Averkin and A. S. Potapov  »View Author Affiliations


Journal of Optical Technology, Vol. 78, Issue 11, pp. 730-734 (2011)
http://dx.doi.org/10.1364/JOT.78.000730


View Full Text Article

Acrobat PDF (523 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

This paper discusses a method of constructing a depth map of a scene from a set of microscope images of the scene. For each coordinate of the image field, the number of the layer in which the variance over a window of definite size takes its maximum value is determined. It is proposed to preprocess the images before the stage of computing the variance, and the operation of separating the image of the object from the background is to be carried out by means of an adaptive threshold with respect to the difference of the maximum and minimum variance over all the layers for each pixel separately. Experimental studies show that the proposed method is suitable for constructing depth maps of various microscopic objects.

© 2011 OSA

History
Original Manuscript: June 6, 2011
Published: November 30, 2011

Citation
A. N. Averkin and A. S. Potapov, "Using the method of depth reconstruction from focusing for microscope images," J. Opt. Technol. 78, 730-734 (2011)
http://www.opticsinfobase.org/jot/abstract.cfm?URI=jot-78-11-730

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited