OSA's Digital Library

Journal of Optical Technology

Journal of Optical Technology


  • Vol. 78, Iss. 11 — Nov. 1, 2011
  • pp: 730–734

Using the method of depth reconstruction from focusing for microscope images

A. N. Averkin and A. S. Potapov  »View Author Affiliations

Journal of Optical Technology, Vol. 78, Issue 11, pp. 730-734 (2011)

View Full Text Article

Acrobat PDF (523 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



This paper discusses a method of constructing a depth map of a scene from a set of microscope images of the scene. For each coordinate of the image field, the number of the layer in which the variance over a window of definite size takes its maximum value is determined. It is proposed to preprocess the images before the stage of computing the variance, and the operation of separating the image of the object from the background is to be carried out by means of an adaptive threshold with respect to the difference of the maximum and minimum variance over all the layers for each pixel separately. Experimental studies show that the proposed method is suitable for constructing depth maps of various microscopic objects.

© 2011 OSA

Original Manuscript: June 6, 2011
Published: November 30, 2011

A. N. Averkin and A. S. Potapov, "Using the method of depth reconstruction from focusing for microscope images," J. Opt. Technol. 78, 730-734 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset


  1. A. P. Pentland, “Depth of scene from depth of field,” in Proc. Image-Understanding Workshop, 1982, pp. 253–259.
  2. M. Niederoest, J. Niederoest, and J. Skucka, “Automatic 3D reconstruction and visualization of microscopic objects from a monoscopic multifocus image sequence,” in ISPRS Archives, XXXIV-5/W10 (2003).
  3. G. L. Greenberg and A. Boyde, “Direct-view 3-D microscopy using conventional lenses,” Eur. Microsc. Analysis 5, (1997).
  4. D. Schmitz, D. Seibt, R. Herpers, and W. Heiden, “3D-reconstruction of microscopic translucent silicate-based marine and freshwater organisms,” in Aktuelle Methoden der Laser- und Medizinphysik: Tagungsband der 2. Remagener Physiktage (2004), pp. 43–48.
  5. G. Packroff, J. R. Lawrence, and T. R. Neu, “In-situ confocal laser scanning microscopy of protozoans in cultures and complex biofilm communities,” Acta Protozool. 41, 245 (2002).
  6. V. Gaganov and A. Ignatenko, “Robust shape from focus via Markov random fields,” in Proc. Graphicon Conf., 2009, pp. 74–80.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited