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Journal of Optical Technology

Journal of Optical Technology


  • Vol. 81, Iss. 1 — Jan. 1, 2014
  • pp: 29–32

Fiber fluorescence–reflection spectrometer with multiwave excitation

G. V. Papayan, N. N. Petrishchev, V. M. Zhurba, A. A. Kishalov, and M. M. Galagudza  »View Author Affiliations

Journal of Optical Technology, Vol. 81, Issue 1, pp. 29-32 (2014)

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A multipurpose fiber fluorescence–reflection spectrometer with multiwave excitation is intended for biomedical studies, including studies in autofluorescence light. For this use, it is equipped with an LED-based illuminating system possessing a number of technical features: fluorescence excitation by radiation with wavelengths 365, 405, and 450 nm, synchronized switching of blocking filters at the excitation wavelength, and rapid switching of illumination with white light and the exciting radiation. This device makes it possible to observe changes of living objects from diffuse reflection and fluorescence spectra recorded virtually simultaneously. The possibilities of the device are illustrated by using it to study how the autofluorescence-excitation conditions affect the metabolic status of myocardium.

© 2014 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics

Original Manuscript: November 5, 2013
Published: March 6, 2014

G. V. Papayan, N. N. Petrishchev, V. M. Zhurba, A. A. Kishalov, and M. M. Galagudza, "Fiber fluorescence–reflection spectrometer with multiwave excitation," J. Opt. Technol. 81, 29-32 (2014)

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  1. V. B.  Loschenov, V. I.  Konov, A. M.  Prokhorov, “Photodynamic therapy and fluorescence diagnostics,” Laser Phys. 10, 1188 (2000).
  2. N. N.  Zharkova, D. N.  Kozlov, Yu. N.  Polivanov, R. L.  Pykhov, V. V.  Smirnov, “Laser-excited fluorescence spectrometric system for tissue diagnostics,” Proc. SPIE 2328, 196 (1994). [CrossRef]
  3. K. A.  Horvath, K. T.  Schomacker, C. C.  Lee, L. H.  Cohn, “Intraoperative myocardial ischemia detection with laser-induced fluorescence,” J. Thorac. Cardiovasc. Surg. 107, 220 (1994).
  4. H. A.  Zeng, N.  MacKinnon, R. W.  Cline, C. E.  MacAulay, “In vivo fluorescence spectroscopy of the gastrointestinal tract under multiple wavelength excitation,” Proc. SPIE 2926, 4 (1996). [CrossRef]
  5. A.  Zuluaga, U.  Utzinger, A.  Durkin, H.  Fuchs, A.  Gillenwater, R.  Jacob, B.  Kemp, J.  Fan, R.  Richards-Kortum, “Fluorescence excitation-emission matrices of human tissue: a system for in vivo measurement and data analysis,” Appl. Spectrosc. 53, 302 (1999). [CrossRef]
  6. K.  Uk, G. V.  Papayan, B. B.  Berezin, N. N.  Petrishchev, M. M.  Galagudza, “Spectrometer for fluorescence–reflection biomedical research,” Opt. Zh. 80, No. 1, 56 (2013) [J. Opt. Technol. 80, 40 (2013)].
  7. B.  Chance, B.  Schoener, R.  Oshino, F.  Itshak, “Oxidation–reduction ratio studies of NADH and flavoprotein fluorescence mitochondria in freeze-trapped samples signals,” J. Biol. Chem. 254, 4764 (1979).
  8. S. M.  Minasyan, M. M.  Galagudza, D. L.  Sonin, D. A.  Zverev, D. V.  Korolyëv, Yu. V.  Dmitriev, M. S.  Vasil’eva, Yu. N.  Grigorova, T. D.  Vlasov, “Technique for perfusing an isolated rat heart,” Region. Krov. Shchenie Mikrotsirk. 8, No. 4, 54 (2009).

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