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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 13 — Jun. 28, 2004
  • pp: 2844–2850

Confocal laser scanning fluorescence microscopy with a visible continuum source

Gail McConnell  »View Author Affiliations

Optics Express, Vol. 12, Issue 13, pp. 2844-2850 (2004)

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Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.

© 2004 Optical Society of America

OCIS Codes
(060.4370) Fiber optics and optical communications : Nonlinear optics, fibers
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(190.4370) Nonlinear optics : Nonlinear optics, fibers

ToC Category:
Research Papers

Original Manuscript: May 13, 2004
Revised Manuscript: June 11, 2004
Published: June 28, 2004

Gail McConnell, "Confocal laser scanning fluorescence microscopy with a visible continuum source," Opt. Express 12, 2844-2850 (2004)

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