Abstract

Collagen, as the most abundant protein in the human body, determines the unique physiological and optical properties of the connective tissues including cornea and sclera. The ultrastructure of collagen, which conventionally can only be resolved by electron microscopy, now can be probed by optical second harmonic generation (SHG) imaging. SHG imaging revealed that corneal collagen fibrils are regularly packed as a polycrystalline lattice, accounting for the transparency of cornea. In contrast, scleral fibrils possess inhomogeneous, tubelike structures with thin hard shells, maintaining the high stiffness and elasticity of the sclera.

© 2005 Optical Society of America

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History
Original Manuscript: May 25, 2005
Revised Manuscript: July 8, 2005
Published: July 25, 2005

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Author Affiliations

Günter Giese

Max-Planck-Institut für medizinische Forschung

Meng Han, Josef Bille

Universität Heidelberg

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