OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 10, Iss. 17 — Aug. 26, 2002
  • pp: 893–898

Axial superresolution with ultrahigh aperture lenses

Carlo Mar Blanca and Stefan W. Hell  »View Author Affiliations


Optics Express, Vol. 10, Issue 17, pp. 893-898 (2002)
http://dx.doi.org/10.1364/OE.10.000893


View Full Text Article

Enhanced HTML    Acrobat PDF (583 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We explore the current limits of the axial resolution of optical sectioning microscopy using a single lens, by combining the resolving power of novel 1.45 numerical aperture oil immersion lenses with superresolving binary aperture filters. We quantify the axial resolution brought about by the increase in semiaperture angle to α max =72.8° and demonstrate an absolute gain in axial resolution through binary pupil filters. Implemented in a confocalized two-photon excitation microscope, the combination of both effectively improves the axial resolution to 330 nm full-width-half-maximum, which is by 34% over that of a standard 1.4 NA system.

© 2002 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Research Papers

History
Original Manuscript: June 4, 2002
Revised Manuscript: August 21, 2002
Published: August 26, 2002

Citation
Carlo Blanca and Stefan Hell, "Axial superresolution with ultrahigh aperture lenses," Opt. Express 10, 893-898 (2002)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-10-17-893


Sort:  Journal  |  Reset  

References

  1. T. Wilson and C. J. R. Sheppard, Theory and Practice of Scanning Optical Microscopy (Academic Press, New York, 1984).
  2. A. Drechsler, M. A. Lieb, C. Debus, A. J. Meixner and G. Tarrach, �??Confocal microscopy with a high numerical aperture parabolic mirror, �?? Opt. Express 9, 637-644 (2001), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-9-12-637">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-9-12-637</a> [CrossRef] [PubMed]
  3. C. M. Blanca, J. Bewersdorf and S. W. Hell, �??Single sharp spot in fluorescence microscopy of two opposing lenses, �?? Appl. Phys. Lett. 79, 2321-2323 (2001). [CrossRef]
  4. C. M. Blanca and S. W. Hell, �??Sharp Spherical Focal Spot by Dark Ring 4Pi-Confocal Microscopy, �?? Single Mol. 2, 207-210 (2001). [CrossRef]
  5. M. Kowalczyk, C. J. Zapata-Rodriguez and M. Martinez-Corral, �??Asymmetric apodization in confocal scanning systems, �?? Appl. Opt. 37, 8206-8214 (1998). [CrossRef]
  6. M. Martinez-Corral, P. Andres, C. J. Zapata-Rodriguez and M. Kowalczyk, �??Three-dimensional superresolution by annular binary filters, �?? Opt. Commun. 165, 267-278 (1999). [CrossRef]
  7. M. Martinez-Corral, M. T. Caballero, E. H. K. Stelzer and J. Swoger, �??Tailoring the axial shape of the point spread function using the Toraldo concept, �?? Opt. Express 10, 98 (2002), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-1-98">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-10-1-98</a> [CrossRef] [PubMed]
  8. C. J. R. Sheppard, G. Calvert and M. Wheatland, �??Focal distribution for superresolving toraldo filters,�?? J. Opt. Soc. Am. A 15, 849-856 (1998). [CrossRef]
  9. M. A. A. Neil, R. Juskaitis, T. Wilson, Z. J. Laczik and V. Sarafis, �??Optimized pupil-plane filters for confocal microscope point-spread function engineering,�?? Opt. Lett. 25, 245-247 (2000). [CrossRef]
  10. B. Richards and E. Wolf, �??Electromagnetic diffraction in opticel systems II. Structure of the image field in an aplanatic system, �?? Proc. R. Soc. Lond. A 253, 358-379 (1959). [CrossRef]
  11. T. Wilson and R. Juskaitis, �??The axial response of confocal microscopes with high numerical aperture objective lenses,�?? Bioimaging 3, 35-38 (1995). [CrossRef]
  12. N. Huse, A. Schönle and S. W. Hell, �??Z-polarized confocal microscopy,�?? J. Biomed. Opt. 6, 480-484 (2001). [CrossRef]
  13. L. Novotny, M. R. Beversluis, K. S. Youngworth and T. G. Brown, �??Longitudinal Field Modes Probed by Single Molecules,�?? Phys. Rev. Lett. 86, 5251-5254 (2001). [CrossRef] [PubMed]
  14. S. Quabis, R. Dorn, M. Eberler, O. Glöckl and G. Leuchs, �??Focusing light to a tighter spot,�?? Opt. Commun. 179, 1-7 (2000). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited