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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 14 — Jul. 14, 2003
  • pp: 1650–1658

High speed, wide velocity dynamic range Doppler optical coherence tomography (Part II): Imaging in vivo cardiac dynamics of Xenopus laevis

Victor X.D. Yang, Maggie L. Gordon, Emily Seng-Yue, Stewart Lo, Bing Qi, Julius Pekar, Alvin Mok, Brian C. Wilson, and I. Alex Vitkin  »View Author Affiliations

Optics Express, Vol. 11, Issue 14, pp. 1650-1658 (2003)

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We previously reported a Doppler optical coherence tomography (DOCT) system design [1] for high-speed imaging with wide velocity dynamic range (up to 28.5 dB when acquiring 8 frames per second), operating at 1.3 µm with a coherence length of 13.5 µm. Using a developmental biology model (Xenopus laevis), here we test the DOCT system’s ability to image cardiac dynamics in an embryo in vivo, with a simple hand-held scanner at 4 ~ 16 frames per second. In particular, we show that high fidelity DOCT movies can be obtained by increasing the reference arm scanning rate (~8 kHz). Utilizing a combination of four display modes (B-mode, color-Doppler, velocity variance, and Doppler spectrum), we show that DOCT can detect changes in velocity distribution during heart cycles, measure the velocity gradient in the embryo, and distinguish blood flow Doppler signal from heart wall motions.

© 2003 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(110.4500) Imaging systems : Optical coherence tomography
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Research Papers

Original Manuscript: May 29, 2003
Revised Manuscript: June 30, 2003
Published: July 14, 2003

Victor X.D. Yang, Maggie Gordon, Emily Seng-Yue, Stewart Lo, Bing Qi, Julius Pekar, Alvin Mok, Brian Wilson, and I. Vitkin, "High speed, wide velocity dynamic range Doppler optical coherence tomography (Part II): Imaging in vivo cardiac dynamics of Xenopus laevis," Opt. Express 11, 1650-1658 (2003)

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  1. V. X. Yang, M. L. Gordon, B. Qi, J. Pekar, S. Lo, E. Seng-Yue, A. Mok, B. C. Wilson, and I. A. Vitkin, "High speed, wide velocity dynamic range Doppler optical coherence tomography (Part I): System design, signal processing, and performance," Opt. Express 11, 794-809 (2003), <a href="http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-7-794.">http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-7-794</a>. [CrossRef] [PubMed]
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