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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 11, Iss. 25 — Dec. 15, 2003
  • pp: 3490–3497

In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical Doppler tomography

Brian R. White, Mark C. Pierce, Nader Nassif, Barry Cense, B. Hyle Park, Guillermo J. Tearney, Brett E. Bouma, Teresa C. Chen, and Johannes F. de Boer  »View Author Affiliations


Optics Express, Vol. 11, Issue 25, pp. 3490-3497 (2003)
http://dx.doi.org/10.1364/OE.11.003490


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Abstract

An ultra-high-speed spectral domain optical Doppler tomography (SD-ODT) system is used to acquire images of blood flow in a human retina in vivo, at 29,000 depth profiles (A-lines) per second and with data acquisition over 99% of the measurement time. The phase stability of the system is examined and image processing algorithms are presented that allow accurate determination of bi-directional Doppler shifts. Movies are presented of human retinal flow acquired at 29 frames per second with 1000 A-lines per frame over a time period of 3.28 seconds, showing accurate determination of vessel boundaries and time-dependent bi-directional flow dynamics in artery-vein pairs. The ultra-high-speed SD-ODT system allows visualization of the pulsatile nature of retinal blood flow, detects blood flow within the choroid and retinal capillaries, and provides information on the cardiac cycle. In summary, accurate video rate imaging of retinal blood flow dynamics is demonstrated at ocular exposure levels below 600 µW.

© 2003 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(170.4470) Medical optics and biotechnology : Ophthalmology
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(280.2490) Remote sensing and sensors : Flow diagnostics

ToC Category:
Research Papers

History
Original Manuscript: November 4, 2003
Revised Manuscript: December 4, 2003
Published: December 15, 2003

Citation
Brian White, Mark Pierce, Nader Nassif, Barry Cense, B. Park, Guillermo Tearney, Brett Bouma, Teresa Chen, and Johannes de Boer, "In vivo dynamic human retinal blood flow imaging using ultra-high-speed spectral domain optical coherence tomography," Opt. Express 11, 3490-3497 (2003)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-11-25-3490


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