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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 11 — May. 31, 2004
  • pp: 2478–2486

In vivo multimodal nonlinear optical imaging of mucosal tissue

Ju Sun, Tuya Shilagard, Brent Bell, Massoud Motamedi, and Gracie Vargas  »View Author Affiliations

Optics Express, Vol. 12, Issue 11, pp. 2478-2486 (2004)

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We present a multimodal nonlinear imaging approach to elucidate microstructures and spectroscopic features of oral mucosa and submucosa in vivo. The hamster buccal pouch was imaged using 3-D high resolution multiphoton and second harmonic generation microscopy. The multimodal imaging approach enables colocalization and differentiation of prominent known spectroscopic and structural features such as keratin, epithelial cells, and submucosal collagen at various depths in tissue. Visualization of cellular morphology and epithelial thickness are in excellent agreement with histological observations. These results suggest that multimodal nonlinear optical microscopy can be an effective tool for studying the physiology and pathology of mucosal tissue.

© 2004 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

Original Manuscript: April 27, 2004
Revised Manuscript: May 21, 2004
Published: May 30, 2004

Ju Sun, Tuya Shilagard, Brent Bell, Massoud Motamedi, and Gracie Vargas, "In vivo multimodal nonlinear optical imaging of mucosal tissue," Opt. Express 12, 2478-2486 (2004)

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