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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 12, Iss. 13 — Jun. 28, 2004
  • pp: 3011–3017

Femtosecond optical tweezers for in-situ control of two-photon fluorescence

B. Agate, C. T. A. Brown, W. Sibbett, and K. Dholakia  »View Author Affiliations


Optics Express, Vol. 12, Issue 13, pp. 3011-3017 (2004)
http://dx.doi.org/10.1364/OPEX.12.003011


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Abstract

We perform a comparison of optical tweezing using continuous wave (cw) and femtosecond lasers. Measurement of the relative Q-values in the femtosecond and cw regimes shows that femtosecond optical tweezers are just as effective as cw optical tweezers. We also demonstrate simultaneous optical tweezing and in-situ control of two-photon fluorescence (at 400nm) from dye-doped polymer microspheres. By switching the 800 nm tweezing laser source between femtosecond and cw regimes, we turned the fluorescent signal from the tweezed particle on and off while maintaining an equivalent tweezing action. Femtosecond lasers can thus be used for optical tweezing and simultaneously utilized to induce nonlinear multi-photon processes such as two-photon excitation or even photoporation.

© 2004 Optical Society of America

OCIS Codes
(140.4050) Lasers and laser optics : Mode-locked lasers
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology

ToC Category:
Research Papers

History
Original Manuscript: April 29, 2004
Revised Manuscript: June 21, 2004
Published: June 28, 2004

Citation
B. Agate, C. Brown, W. Sibbett, and K. Dholakia, "Femtosecond optical tweezers for in-situ control of two-photon fluorescence," Opt. Express 12, 3011-3017 (2004)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-12-13-3011


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