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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 10 — May. 16, 2005
  • pp: 3583–3593

Single particle multichannel bio-aerosol fluorescence sensor

P. H. Kaye, W. R. Stanley, E. Hirst, E.V. Foot, K. L. Baxter, and S. J. Barrington  »View Author Affiliations

Optics Express, Vol. 13, Issue 10, pp. 3583-3593 (2005)

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We describe a prototype low-cost multi-channel aerosol fluorescence sensor designed for unattended deployment in medium to large area bio-aerosol detection networks. Individual airborne particles down to ~1µm in size are detected and sized by measurement of light scattered from a continuous-wave diode laser (660nm). This scatter signal is then used to trigger the sequential firing of two xenon sources which irradiate the particle with UV pulses at ~280 nm and ~370 nm, optimal for excitation of bio-fluorophores tryptophan and NADH (nicotinamide adenine dinucleotide) respectively. For each excitation wavelength, fluorescence is detected across two bands embracing the peak emissions of the same bio-fluorophores. Current measurement rates are up to ~125 particles/s, corresponding to all particles for concentrations up to 1.3×104 particles/l. Developments to increase this to ~500 particles/s are in hand. Device sensitivity is illustrated in preliminary data recorded from aerosols of E.coli, BG spores, and a variety of non-biological materials.

© 2005 Optical Society of America

OCIS Codes
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(260.2510) Physical optics : Fluorescence

ToC Category:
Research Papers

Original Manuscript: April 8, 2005
Revised Manuscript: April 26, 2005
Published: May 16, 2005

P. Kaye, W. R. Stanley, E. Hirst, E. V. Foot, K. L. Baxter, and S. J. Barrington, "Single particle multichannel bio-aerosol fluorescence sensor," Opt. Express 13, 3583-3593 (2005)

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