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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 16 — Aug. 8, 2005
  • pp: 6268–6274

Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy

A.-M. Pena, M. Strupler, T. Boulesteix, and M.-C. Schanne-Klein  »View Author Affiliations

Optics Express, Vol. 13, Issue 16, pp. 6268-6274 (2005)

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We recorded one-photon excited fluorescence (1PEF) and two-photon excited fluorescence (2PEF) spectra of purified keratin from human epidermis, and determined the action cross section of this endogenous chromophore. We used this spectroscopic analysis to analyse multiphoton images of skin biopsies and assign the intrinsic fluorescence signals in the epidermis. We observed a good agreement between in situ and in vitro 2PEF spectra of keratin. This study provides a comprehensive characterization of the 2PEF signal of the keratins from the epidermis, and will be of practical interest for multiphoton imaging of the skin.

© 2005 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Research Papers

Original Manuscript: May 25, 2005
Revised Manuscript: July 25, 2005
Published: August 8, 2005

A. Pena, M. Strupler, T. Boulesteix, and M. Schanne-Klein, "Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy," Opt. Express 13, 6268-6274 (2005)

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