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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 18 — Sep. 5, 2005
  • pp: 6738–6749

Extended focused image in microscopy by digital Holography

Pietro Ferraro, Simonetta Grilli, Domenico Alfieri, Sergio De Nicola, Andrea Finizio, Giovanni Pierattini, Bahram Javidi, Giuseppe Coppola, and Valerio Striano  »View Author Affiliations

Optics Express, Vol. 13, Issue 18, pp. 6738-6749 (2005)

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In microscopy, high magnifications are achievable for investigating micro-objects but the paradigm is that higher is the required magnification, lower is the depth of focus. For an object having a three-dimensional (3D) complex shape only a portion of it appears in good focus to the observer who is essentially looking at a single image plane. Actually, two approaches exist to obtain an extended focused image, both having severe limitations since the first requires mechanical scanning while the other one requires specially designed optics. We demonstrate that an extended focused image of an object can be obtained through digital holography without any mechanical scanning or special optical components. The conceptual novelty of the proposed approach lies in the fact that it is possible to completely exploit the unique feature of DH in extracting all the information content stored in hologram, amplitude and phase, to extend the depth of focus.

© 2005 Optical Society of America

OCIS Codes
(070.4560) Fourier optics and signal processing : Data processing by optical means
(090.0090) Holography : Holography
(100.3020) Image processing : Image reconstruction-restoration
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Research Papers

Original Manuscript: June 8, 2005
Revised Manuscript: July 14, 2005
Published: September 5, 2005

Pietro Ferraro, Simonetta Grilli, Domenico Alfieri, Sergio De Nicola, Andrea Finizio, Giovanni Pierattini, Bahram Javidi, Giuseppe Coppola, and Valerio Striano, "Extended focused image in microscopy by digital Holography," Opt. Express 13, 6738-6749 (2005)

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