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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 18 — Sep. 5, 2005
  • pp: 7035–7044

Single molecule fluorescence in rectangular nano-apertures

Jérôme Wenger, Pierre-François Lenne, Evgueni Popov, Hervé Rigneault, José Dintinger, and Thomas Ebbesen  »View Author Affiliations


Optics Express, Vol. 13, Issue 18, pp. 7035-7044 (2005)
http://dx.doi.org/10.1364/OPEX.13.007035


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Abstract

Fluorescence Correlation Spectroscopy is used to investigate fluorescent molecules in solution diffusing in subwavelength rectangular apertures milled in Aluminium films. This rectangular shape allows to switch between a propagating and an evanescent excitation field within the aperture, leading to a significant tunability of the observation volume. Due to the vicinity of the metal surface, the fluorophore�??s molecular lifetime inside the aperture appears to be dramatically reduced whatever the excitation field is set to. However, for a properly tailored evanescent excitation field within the nanoaperture, the detected fluorescence rate per molecule is significantly enhanced as compared to open solution. This suggests that the observed molecular fluorescence enhancement is mainly due to the excitation near field within the subwavelength aperture.

© 2005 Optical Society of America

OCIS Codes
(050.1220) Diffraction and gratings : Apertures
(180.2520) Microscopy : Fluorescence microscopy
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Research Papers

History
Original Manuscript: August 1, 2005
Revised Manuscript: August 25, 2005
Published: September 5, 2005

Citation
Jérôme Wenger, Pierre-François Lenne, Evgueni Popov, Hervé Rigneault, José Dintinger, and Thomas Ebbesen, "Single molecule fluorescence in rectangular nano-apertures," Opt. Express 13, 7035-7044 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-18-7035


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