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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 22 — Oct. 31, 2005
  • pp: 8855–8865

The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection

Jörg Enderlein and Thomas Ruckstuhl  »View Author Affiliations

Optics Express, Vol. 13, Issue 22, pp. 8855-8865 (2005)

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Surface-plasmon coupled emission (SPCE) has emerged as a new and potentially powerful tool for highly sensitive fluorescence detection. In the case of SPCE, the fluorescence is collected through a semi-transparent thin metal film deposited on glass. We present a theoretical analysis of SPCE, studying the potential enhancement of the fluorescence collection efficiency, brightness, quantum-yield, and photostability. The results are compared with fluorescence detection on a pure glass surface. It is shown that SPCE does not lead to any improvement, but that the metal film actually reduces the sensitivity of fluorescence detection.

© 2005 Optical Society of America

OCIS Codes
(240.6680) Optics at surfaces : Surface plasmons
(260.2110) Physical optics : Electromagnetic optics
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Research Papers

Original Manuscript: September 1, 2005
Revised Manuscript: October 18, 2005
Published: October 31, 2005

Jörg Enderlein and Thomas Ruckstuhl, "The efficiency of surface-plasmon coupled emission for sensitive fluorescence detection," Opt. Express 13, 8855-8865 (2005)

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