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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 24 — Nov. 28, 2005
  • pp: 9812–9821

All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

A. Esposito, T. Oggier, H.C. Gerritsen, F. Lustenberger, and F.S. Wouters  »View Author Affiliations

Optics Express, Vol. 13, Issue 24, pp. 9812-9821 (2005)

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Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting- and laser diodes could drive a wider spreading of the use of FLIM including high-throughput applications.

© 2005 Optical Society of America

OCIS Codes
(040.6070) Detectors : Solid state detectors
(150.6910) Machine vision : Three-dimensional sensing
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Research Papers

Original Manuscript: September 8, 2005
Revised Manuscript: September 7, 2005
Published: November 28, 2005

A. Esposito, T. Oggier, H. Gerritsen, F. Lustenberger, and F. Wouters, "All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing," Opt. Express 13, 9812-9821 (2005)

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  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum Publishers, New York 1999).
  2. E. A. Jares-Erijman and T. M. Jovin, "FRET imaging," Nat. Biotechnol. 21, 1387-1395 (2003). [CrossRef] [PubMed]
  3. F. S. Wouters, P. J. Verveer, and P. I. Bastiaens, "Imaging biochemistry inside cells," Trends Cell Biol. 11, 203-211 (2001). [CrossRef] [PubMed]
  4. A. Esposito and F. S. Wouters, "Fluorescence Lifetime Imaging Microscopy" in Current Protocols in Cell Biology, Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, and Kenneth M. Yamada, eds. 2004). [CrossRef]
  5. S. Landgraf, "Application of semiconductor light sources for investigations of photochemical reactions," Spectrochim. Acta A Mol. Biomol. Spectrosc. 57, 2029-2048 (2001). [CrossRef] [PubMed]
  6. A. C. Mitchell, J. E. Wall, J. G. Murray, and C. G. Morgan, "Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging," J. Microsc. 206, 225-232 (2002). [CrossRef] [PubMed]
  7. A. C. Mitchell, J. E. Wall, J. G. Murray, and C. G. Morgan, "Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera," J. Microsc. 206, (2002). [CrossRef] [PubMed]
  8. K. Nishikata, Y. Kimura, Y. Takai, T. Ikuta, and R. Shimizu, "Real-time lock-in imaging by a newly developed high-speed image-processing charged coupled device video camera," Rev. Sci. Instrum. 74, 1393-1396 (2003). [CrossRef]
  9. T. Oggier, M. Lehmann, R. Kaufmann, M. Schweizer, M. Richter, P. Metzler, G. Lang, F. Lustenberger, and N. Blanc, "An all-solid-state optical range camera for 3D real-time imaging with sub-centimeter depth resolution (SwissRanger)," in Optical Design and Engineering, L. Mazuray, P.J. Rogers and R. Wartmann, eds., Proc. SPIE 5249, 534-545 (2004). [CrossRef]
  10. R. Lange, P. Seitz, A. Biber, and R. Schwarte, "Time-of-flight range imaging with a custom solid state image sensor," in Laser Metrology and Inspection, H.J. Tiziani and P.K. Rastogi, eds., Proc. SPIE 3823, 180-191 (1999). [CrossRef]
  11. O. Zapata-Hommer and O. Griesbeck, "Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP," BMC Biotechnol. 3, (2003). [CrossRef] [PubMed]
  12. T. W. Gadella, Jr., T. M. Jovin, and R. M. Clegg, "Fluorescence Lifetime Imaging Microscopy (FLIM) -Spatial-Resolution of Microstructures on the Nanosecond Time-Scale," Biophys. Chem. 48, 221-239 (1993). [CrossRef]
  13. E. B. van Munster and T. W. Gadella, Jr., "Suppression of photobleaching-induced artifacts in frequencydomain FLIM by permutation of the recording order," Cytometry 58A, (2004).
  14. R. M. Clegg and P. C. Schneider, "Fluorescence lifetime-resolved imaging microscopy: a general description of lifetime-resolved imaging measurements" in Fluorescence Microscopy and Fluorescent Probes, J. Slavik, ed. (Plenum Press, New York 1996).
  15. J. Philip and K. Carlsson, "Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging," J. Opt. Soc. Am. A Opt. Image Sci. Vis. 20, (2003). [CrossRef] [PubMed]
  16. H. C. Gerritsen, M. A. Asselbergs, A. V. Agronskaia, and W. G. Van Sark, "Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution," J. Microsc. 206, (2002). [CrossRef] [PubMed]

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