OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 24 — Nov. 28, 2005
  • pp: 9812–9821

All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

A. Esposito, T. Oggier, H.C. Gerritsen, F. Lustenberger, and F.S. Wouters  »View Author Affiliations


Optics Express, Vol. 13, Issue 24, pp. 9812-9821 (2005)
http://dx.doi.org/10.1364/OPEX.13.009812


View Full Text Article

Enhanced HTML    Acrobat PDF (265 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting- and laser diodes could drive a wider spreading of the use of FLIM including high-throughput applications.

© 2005 Optical Society of America

OCIS Codes
(040.6070) Detectors : Solid state detectors
(150.6910) Machine vision : Three-dimensional sensing
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Research Papers

History
Original Manuscript: September 8, 2005
Revised Manuscript: September 7, 2005
Published: November 28, 2005

Citation
A. Esposito, T. Oggier, H. Gerritsen, F. Lustenberger, and F. Wouters, "All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing," Opt. Express 13, 9812-9821 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-24-9812


Sort:  Journal  |  Reset  

References

  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Kluwer Academic/Plenum Publishers, New York 1999).
  2. E. A. Jares-Erijman and T. M. Jovin, "FRET imaging," Nat. Biotechnol. 21, 1387-1395 (2003). [CrossRef] [PubMed]
  3. F. S. Wouters, P. J. Verveer, and P. I. Bastiaens, "Imaging biochemistry inside cells," Trends Cell Biol. 11, 203-211 (2001). [CrossRef] [PubMed]
  4. A. Esposito and F. S. Wouters, "Fluorescence Lifetime Imaging Microscopy" in Current Protocols in Cell Biology, Juan S. Bonifacino, Mary Dasso, Joe B. Harford, Jennifer Lippincott-Schwartz, and Kenneth M. Yamada, eds. 2004). [CrossRef]
  5. S. Landgraf, "Application of semiconductor light sources for investigations of photochemical reactions," Spectrochim. Acta A Mol. Biomol. Spectrosc. 57, 2029-2048 (2001). [CrossRef] [PubMed]
  6. A. C. Mitchell, J. E. Wall, J. G. Murray, and C. G. Morgan, "Direct modulation of the effective sensitivity of a CCD detector: a new approach to time-resolved fluorescence imaging," J. Microsc. 206, 225-232 (2002). [CrossRef] [PubMed]
  7. A. C. Mitchell, J. E. Wall, J. G. Murray, and C. G. Morgan, "Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera," J. Microsc. 206, (2002). [CrossRef] [PubMed]
  8. K. Nishikata, Y. Kimura, Y. Takai, T. Ikuta, and R. Shimizu, "Real-time lock-in imaging by a newly developed high-speed image-processing charged coupled device video camera," Rev. Sci. Instrum. 74, 1393-1396 (2003). [CrossRef]
  9. T. Oggier, M. Lehmann, R. Kaufmann, M. Schweizer, M. Richter, P. Metzler, G. Lang, F. Lustenberger, and N. Blanc, "An all-solid-state optical range camera for 3D real-time imaging with sub-centimeter depth resolution (SwissRanger)," in Optical Design and Engineering, L. Mazuray, P.J. Rogers and R. Wartmann, eds., Proc. SPIE 5249, 534-545 (2004). [CrossRef]
  10. R. Lange, P. Seitz, A. Biber, and R. Schwarte, "Time-of-flight range imaging with a custom solid state image sensor," in Laser Metrology and Inspection, H.J. Tiziani and P.K. Rastogi, eds., Proc. SPIE 3823, 180-191 (1999). [CrossRef]
  11. O. Zapata-Hommer and O. Griesbeck, "Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP," BMC Biotechnol. 3, (2003). [CrossRef] [PubMed]
  12. T. W. Gadella, Jr., T. M. Jovin, and R. M. Clegg, "Fluorescence Lifetime Imaging Microscopy (FLIM) -Spatial-Resolution of Microstructures on the Nanosecond Time-Scale," Biophys. Chem. 48, 221-239 (1993). [CrossRef]
  13. E. B. van Munster and T. W. Gadella, Jr., "Suppression of photobleaching-induced artifacts in frequencydomain FLIM by permutation of the recording order," Cytometry 58A, (2004).
  14. R. M. Clegg and P. C. Schneider, "Fluorescence lifetime-resolved imaging microscopy: a general description of lifetime-resolved imaging measurements" in Fluorescence Microscopy and Fluorescent Probes, J. Slavik, ed. (Plenum Press, New York 1996).
  15. J. Philip and K. Carlsson, "Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging," J. Opt. Soc. Am. A Opt. Image Sci. Vis. 20, (2003). [CrossRef] [PubMed]
  16. H. C. Gerritsen, M. A. Asselbergs, A. V. Agronskaia, and W. G. Van Sark, "Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution," J. Microsc. 206, (2002). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited