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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 13, Iss. 24 — Nov. 28, 2005
  • pp: 9869–9880

Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator

Tomoko Shimada, Wataru Watanabe, Sachihiro Matsunaga, Tsunehito Higashi, Hiroshi Ishii, Kiichi Fukui, Keisuke Isobe, and Kazuyoshi Itoh  »View Author Affiliations

Optics Express, Vol. 13, Issue 24, pp. 9869-9880 (2005)

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Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The long-term viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability.

© 2005 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:
Research Papers

Original Manuscript: October 18, 2005
Revised Manuscript: October 18, 2005
Published: November 28, 2005

Tomoko Shimada, Wataru Watanabe, Sachihiro Matsunaga, Tsunehito Higashi, Hiroshi Ishii, Kiichi Fukui, Keisuke Isobe, and Kazuyoshi Itoh, "Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator," Opt. Express 13, 9869-9880 (2005)

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