Spiral phase contrast imaging in microscopy
Optics Express, Vol. 13, Issue 3, pp. 689-694 (2005)
http://dx.doi.org/10.1364/OPEX.13.000689
Enhanced HTML
Acrobat PDF (113 KB)
Abstract
We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(iφ) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.
© 2005 Optical Society of America
OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(090.1970) Holography : Diffractive optics
(100.5090) Image processing : Phase-only filters
ToC Category:
Research Papers
History
Original Manuscript: December 1, 2004
Revised Manuscript: January 10, 2005
Published: February 7, 2005
Citation
Severin Fürhapter, Alexander Jesacher, Stefan Bernet, and Monika Ritsch-Marte, "Spiral phase contrast imaging in microscopy," Opt. Express 13, 689-694 (2005)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-13-3-689
Sort: Journal | Reset
References
- N. R. Heckenberg, R. McDuff, C. P. Smith, and A. G. White, �??Generation of optical phase singularities by computer-generated holograms,�?? Opt. Lett. 17, 221�??223 (1992) [CrossRef] [PubMed]
- R. Oron, N. Davidson, A. A. Friesem, and E. Hasman, �??Transverse mode shaping and selection in laser resonators,�?? Progress in Optics 42, 325�??386 (2001) [CrossRef]
- S. S. R. Oemrawsingh, J. A.W. van Houwelingen, E. R. Eliel, J. P.Woerdman, E. J. K. Verstegen, J. G. Kloosterboer, and G. W. �??t Hooft, �??Production and characterization of spiral phase plates for optical wavelengths,�?? Appl. Opt. 43, 688�??694 (2004) [CrossRef] [PubMed]
- A. Y. M. NG, C. W. See, and M. G. Somekh, �??Quantitative optical microscope with enhanced resolution using a pixelated liquid crystal spatial light modulator,�?? J. Microscopy 214, 334�??340 (2004) [CrossRef]
- J. A. Davis, D. E. McNamara, D. M. Cottrell, and J. Campos, �??Image processing with the radial Hilbert transform: theory and experiments,�?? Opt. Lett. 25, 99�??101 (2000) [CrossRef]
- K. Crabtree, J. A. Davis, and I. Moreno, �??Optical processing with vortex-producing lenses,�?? Appl. Opt. 43, 1360�??1367 (2004) [CrossRef] [PubMed]
- G. A. Swartzlander, Jr., �??Peering into darkness with a vortex spatial filter,�?? Opt. Lett. 26, 497�??499 (2001) [CrossRef]
- M. R. Arnison, K. G. Larkin, C. J. R. Sheppard, N. I. Smith, and C. J. Cogswell, �??Linear phase imaging using differential interference contrast microscopy,�?? J. Microscopy 214, 7�??12 (2004) [CrossRef]
- G. O. Reynolds, J. B. DeVelis, G. B. Parrent, Jr., and B. J. Thompson, The new physical optics notebook: Tutorials in Fourier optics (SPIE Optical Engineering Press, Bellingham, Washington, 1989) [CrossRef]
- K. G. Larkin, D. J. Bone, and M. A. Oldfield, �??Natural demodulation of two-dimensional fringe patterns. I. General background of the spiral phase quadrature transform,�?? J. Opt. Soc. Am. A, 18, 1862�??1870 (2001) [CrossRef]
- A. Jesacher, S. F¨urhapter, S. Bernet, and M. Ritsch-Marte , �??Diffractive optical tweezers in the Fresnel regime,�?? Opt. Express 12, 2243-2250 (2004), //www.opticsexpress.org/abstract.cfm?URI=OPEX-12-10-2243 [CrossRef] [PubMed]
Cited By |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Multimedia
| Multimedia Files | Recommended Software |
| » Media 1: MPG (1037 KB) |





OSA is a member of 