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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 10 — May. 15, 2006
  • pp: 4300–4306

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Tristan Colomb, Jonas Kühn, Florian Charrière, Christian Depeursinge, Pierre Marquet, and Nicolas Aspert  »View Author Affiliations


Optics Express, Vol. 14, Issue 10, pp. 4300-4306 (2006)
http://dx.doi.org/10.1364/OE.14.004300


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Abstract

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram. We demonstrate that in particular cases in which the studied specimen does not have abrupt edges, the specimen’s hologram itself can be used as reference hologram. We show that image distortion and phase aberrations introduced by a lens ball used as microscope objective are completely suppressed with our method. Finally the concept of self-conjugated reference hologram is applied on a biological sample (Trypanosoma Brucei) to maintain a spatial phase noise level under 3 degrees.

© 2006 Optical Society of America

OCIS Codes
(090.1000) Holography : Aberration compensation
(090.1760) Holography : Computer holography
(180.3170) Microscopy : Interference microscopy

ToC Category:
Holography

History
Original Manuscript: March 21, 2006
Revised Manuscript: May 3, 2006
Manuscript Accepted: May 5, 2006
Published: May 15, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Tristan Colomb, Jonas Kühn, Florian Charrière, Christian Depeursinge, Pierre Marquet, and Nicolas Aspert, "Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram," Opt. Express 14, 4300-4306 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-10-4300


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References

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