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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7134–7143

Fluorescence coherence tomography

A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney  »View Author Affiliations

Optics Express, Vol. 14, Issue 16, pp. 7134-7143 (2006)

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In this paper, we introduce a new form of cross-sectional, coherence-gated fluorescence imaging, which we term ‘spectral-domain fluorescence coherence tomography’ (SD-FCT). SD-FCT is accomplished by spectrally detecting self-interference of the spontaneous emission of fluorophores located along the axial (depth) dimension of the sample. We have built a first generation SD-FCT system that utilizes two opposing low numerical-aperture objective lenses in an interferometer and an imaging spectrometer for detecting self-interference of fluorescence emitted from a sample. Here, in proof-of-principle experiments we demonstrate cross-sectional profiling of layered fluorescence phantoms. Narrow (a few micrometers FWHM) axial point-spread functions, large ranging depths (a few hundreds of micrometers) and wide fields of view (>1 mm) were measured. Initial results suggest that SD-FCT may be a viable tool for the investigation of semi-transparent and selectively labeled fluorescent samples.

© 2006 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(170.6960) Medical optics and biotechnology : Tomography
(260.2510) Physical optics : Fluorescence

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 13, 2006
Revised Manuscript: July 19, 2006
Manuscript Accepted: July 25, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney, "Fluorescence coherence tomography," Opt. Express 14, 7134-7143 (2006)

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  1. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science 254, 1178-1181 (1991). [CrossRef] [PubMed]
  2. A. F. Fercher, W. Drexler, C. K. Hitzenberger, and T. Lasser, "Optical coherence tomography - principles and applications," Rep. Prog. Phys. 66, 239-303 (2003). [CrossRef]
  3. J. A. Izatt, M. R. Hee, G. M. Owen, E. A. Swanson, and J. G. Fujimoto, "Optical coherence microscopy in scattering media," Opt. Lett. 19, 590-592 (1994). [CrossRef] [PubMed]
  4. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, "Optical sectioning deep inside live embryos by selective plane illumination microscopy," Science 305, 1007-1009 (2004). [CrossRef] [PubMed]
  5. K. H. Drexhage, "Interaction of light with monomolecular dye layers," Prog. Opt. 12, 163-232 (1974). [CrossRef]
  6. K. E. Drabe, G. Cnossen, and D. A. Wiersma, "Localization of spontaneous emission in front of a mirror," Opt. Commun. 73, 91-95 (1989). [CrossRef]
  7. A. Lambacher and P. Fromherz, "Fluorescence interference-contrast microscopy on oxidized silicon using a monomolecular dye layer," Appl. Phys. A 63, 207-216 (1996). [CrossRef]
  8. A. K. Swan, L. A. Moiseev, C. R. Cantor, B. Davis, S. B. Ippolito, W. C. Karl, B. B. Goldberg, M. S. Unlu, "Toward nanometer-scale resolution in fluorescence microscopy using spectral self-interference," IEEE J. Sel. Top. Quantum Electron. 9, 294-300 (2003). [CrossRef]
  9. S. Hell and E. H. K. Stelzer, "Properties of a 4Pi-confocal fluorescence microscope," J. Opt. Soc. Am. A 9, 2159-2166 (1992). [CrossRef]
  10. M. G. L. Gustafsson, D. A. Agard and J. W. Sedat, "Sevenfold improvement of axial resolution in 3D widefiled microscopy using two objective lenses," in Three-dimensional Microscopy: Image Acquisition and Processing II, Tony Wilson and Carol J. Cogswell, eds., 2412, 147-156 (1995).
  11. B. Karamata, P. Lambelet, M. Laubscher, R. P. Salathé, and T. Lasser, "Spatially incoherent illumination as a mechanism for cross-talk suppression in wide-field optical coherence tomography," Opt. Lett. 29, 736-738 (2004). [CrossRef] [PubMed]
  12. M. Wojtkowski, A. Kowalczyk, R. Leitgeb, and A. F. Fercher, "Full range complex spectral optical coherence tomography technique in eye imaging," Opt. Lett. 27, 1415-1417 (2002). [CrossRef]
  13. B. Grajciar, M. Pircher, A. Fercher, and R. Leitgeb, "Parallel Fourier domain optical coherence tomography for in vivo measurement of the human eye," Opt. Express 13, 1131-1137 (2005). [CrossRef] [PubMed]
  14. C. Dorrer, N. Belabas, J-P Likforman, and M. Joffre, "Spectral resolution and sampling issues in Fourier transform spectral interferometry," J. Opt. Soc. Am. B 17, 1795-1802 (2000). [CrossRef]
  15. N. Nassif, B. Cense, B. Park, M. Pierce, S. Yun, B. Bouma, G. Tearney, T. Chen, and J. de Boer, "In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve," Opt. Express 12, 367-376 (2004). [CrossRef] [PubMed]
  16. S. Yun, G. Tearney, B. Bouma, B. Park, and J. de Boer, "High-speed spectral-domain optical coherence tomography at 1.3 μm wavelength," Opt. Express 11, 3598-3604 (2003). [CrossRef] [PubMed]
  17. A. Bilenca, A. Desjardins, B. Bouma, and G. Tearney, "Multicanonical Monte-Carlo simulations of light propagation in biological media," Opt. Express 13, 9822-9833 (2005). [CrossRef] [PubMed]

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