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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7134–7143

Fluorescence coherence tomography

A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney  »View Author Affiliations


Optics Express, Vol. 14, Issue 16, pp. 7134-7143 (2006)
http://dx.doi.org/10.1364/OE.14.007134


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Abstract

In this paper, we introduce a new form of cross-sectional, coherence-gated fluorescence imaging, which we term ‘spectral-domain fluorescence coherence tomography’ (SD-FCT). SD-FCT is accomplished by spectrally detecting self-interference of the spontaneous emission of fluorophores located along the axial (depth) dimension of the sample. We have built a first generation SD-FCT system that utilizes two opposing low numerical-aperture objective lenses in an interferometer and an imaging spectrometer for detecting self-interference of fluorescence emitted from a sample. Here, in proof-of-principle experiments we demonstrate cross-sectional profiling of layered fluorescence phantoms. Narrow (a few micrometers FWHM) axial point-spread functions, large ranging depths (a few hundreds of micrometers) and wide fields of view (>1 mm) were measured. Initial results suggest that SD-FCT may be a viable tool for the investigation of semi-transparent and selectively labeled fluorescent samples.

© 2006 Optical Society of America

OCIS Codes
(030.1670) Coherence and statistical optics : Coherent optical effects
(170.6960) Medical optics and biotechnology : Tomography
(260.2510) Physical optics : Fluorescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 13, 2006
Revised Manuscript: July 19, 2006
Manuscript Accepted: July 25, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
A. Bilenca, A. Ozcan, B. Bouma, and G. Tearney, "Fluorescence coherence tomography," Opt. Express 14, 7134-7143 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-16-7134


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