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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 16 — Aug. 7, 2006
  • pp: 7144–7158

In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells

Daniel C. Gray, William Merigan, Jessica I. Wolfing, Bernard P. Gee, Jason Porter, Alfredo Dubra, Ted H. Twietmeyer, Kamran Ahmad, Remy Tumbar, Fred Reinholz, and David R. Williams  »View Author Affiliations


Optics Express, Vol. 14, Issue 16, pp. 7144-7158 (2006)
http://dx.doi.org/10.1364/OE.14.007144


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Abstract

The ability to resolve single cells noninvasively in the living retina has important applications for the study of normal retina, diseased retina, and the efficacy of therapies for retinal disease. We describe a new instrument for high-resolution, in vivo imaging of the mammalian retina that combines the benefits of confocal detection, adaptive optics, multispectral, and fluorescence imaging. The instrument is capable of imaging single ganglion cells and their axons through retrograde transport in ganglion cells of fluorescent dyes injected into the monkey lateral geniculate nucleus (LGN). In addition, we demonstrate a method involving simultaneous imaging in two spectral bands that allows the integration of very weak signals across many frames despite inter-frame movement of the eye. With this method, we are also able to resolve the smallest retinal capillaries in fluorescein angiography and the mosaic of retinal pigment epithelium (RPE) cells with lipofuscin autofluorescence.

© 2006 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(330.4460) Vision, color, and visual optics : Ophthalmic optics and devices

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 23, 2006
Revised Manuscript: July 17, 2006
Manuscript Accepted: July 19, 2006
Published: August 7, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Daniel C. Gray, William Merigan, Jessica I. Wolfing, Bernard P. Gee, Jason Porter, Alfredo Dubra, Ted H. Twietmeyer, Kamran Ahamd, Remy Tumbar, Fred Reinholz, and David R. Williams, "In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells," Opt. Express 14, 7144-7158 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-16-7144


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