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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 17 — Aug. 21, 2006
  • pp: 7789–7800

In vivo imaging flow cytometer

Ho Lee, Clemens Alt, Costas M. Pitsillides, Mehron Puoris’haag, and Charles P. Lin  »View Author Affiliations

Optics Express, Vol. 14, Issue 17, pp. 7789-7800 (2006)

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We introduce an in vivo imaging flow cytometer, which provides fluorescence images simultaneously with quantitative information on the cell population of interest in a live animal. As fluorescent cells pass through the slit of light focused across a blood vessel, the excited fluorescence is confocally detected. This cell signal triggers a strobe beam and a high sensitivity CCD camera that captures a snapshot image of the cell as it moves down-stream from the slit. We demonstrate that the majority of signal peaks detected in the in vivo flow cytometer arise form individual cells. The instrument’s capability to image circulating T cells and measure their speed in the blood vessel in real time in vivo is demonstrated. The cell signal irradiance variation, clustering percentage, and potential applications in biology and medicine are discussed.

© 2006 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 4, 2006
Revised Manuscript: June 29, 2006
Manuscript Accepted: July 20, 2006
Published: August 21, 2006

Virtual Issues
Vol. 1, Iss. 9 Virtual Journal for Biomedical Optics

Ho Lee, Clemens Alt, Costas M. Pitsillides, Mehron Puoris'haag, and Charles P. Lin, "In vivo imaging flow cytometer," Opt. Express 14, 7789-7800 (2006)

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  1. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, "In vivo flow cytometer for real-time detection and quantification of circulating cells," Opt. Lett. 29, 77-79 (2004). [CrossRef] [PubMed]
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