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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 2 — Jan. 23, 2006
  • pp: 956–969

Confining the sampling volume for Fluorescence Correlation Spectroscopy using a sub-wavelength sized aperture

Marcel Leutenegger, Michael Gösch, Alexandre Perentes, Patrik Hoffmann, Olivier J.F. Martin, and Theo Lasser  »View Author Affiliations


Optics Express, Vol. 14, Issue 2, pp. 956-969 (2006)
http://dx.doi.org/10.1364/OPEX.14.000956


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Abstract

For the observation of single molecule dynamics with fluorescence fluctuation spectroscopy (FFS) very low fluorophore concentrations are necessary. For in vitro measurements, this requirement is easy to fulfill. In biology however, micromolar concentrations are often encountered and may pose a real challenge to conventional FFS methods based on confocal instrumentation. We show a higher confinement of the sampling volume in the near-field of sub-wavelength sized apertures in a thin gold film. The gold apertures have been measured and characterized with fluorescence correlation spectroscopy (FCS), indicating light confinement beyond the far-field diffraction limit. We measured a reduction of the effective sampling volume by an order of magnitude compared to confocal instrumentation.

© 2006 Optical Society of America

OCIS Codes
(050.1220) Diffraction and gratings : Apertures
(160.3900) Materials : Metals
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(240.6490) Optics at surfaces : Spectroscopy, surface
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Spectroscopy

Virtual Issues
Vol. 1, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Marcel Leutenegger, Michael Gösch, Alexandre Perentes, Patrik Hoffmann, Olivier J. F. Martin, and Theo Lasser, "Confining the sampling volume for Fluorescence Correlation Spectroscopy using a sub-wavelength sized aperture," Opt. Express 14, 956-969 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-2-956


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