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Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 8 — Apr. 17, 2006
  • pp: 3406–3414

Stochastic scanning multiphoton multifocal microscopy

Justin E. Jureller, Hee Y. Kim, and Norbert F. Scherer  »View Author Affiliations

Optics Express, Vol. 14, Issue 8, pp. 3406-3414 (2006)

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Multiparticle tracking with scanning confocal and multiphoton fluorescence imaging is increasingly important for elucidating biological function, as in the transport of intracellular cargo-carrying vesicles. We demonstrate a simple rapid-sampling stochastic scanning multiphoton multifocal microscopy (SS-MMM) fluorescence imaging technique that enables multiparticle tracking without specialized hardware at rates 1,000 times greater than conventional single point raster scanning. Stochastic scanning of a diffractive optic generated 10x10 hexagonal array of foci with a white noise driven galvanometer yields a scan pattern that is random yet space-filling. SS-MMM creates a more uniformly sampled image with fewer spatio-temporal artifacts than obtained by conventional or multibeam raster scanning. SS-MMM is verified by simulation and experimentally demonstrated by tracking microsphere diffusion in solution.

© 2006 Optical Society of America

OCIS Codes
(050.1970) Diffraction and gratings : Diffractive optics
(110.0180) Imaging systems : Microscopy
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:

Original Manuscript: February 28, 2006
Revised Manuscript: April 11, 2006
Manuscript Accepted: April 12, 2006
Published: April 17, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Justin E. Jureller, Hee Y. Kim, and Norbert F. Scherer, "Stochastic scanning multiphoton multifocal microscopy," Opt. Express 14, 3406-3414 (2006)

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