OSA's Digital Library

Optics Express

Optics Express

  • Editor: Michael Duncan
  • Vol. 14, Iss. 9 — May. 1, 2006
  • pp: 3952–3960

High-speed autofocusing of a cell using diffraction patterns

Hiromasa Oku, Masatoshi Ishikawa, Theodorus, and Koichi Hashimoto  »View Author Affiliations


Optics Express, Vol. 14, Issue 9, pp. 3952-3960 (2006)
http://dx.doi.org/10.1364/OE.14.003952


View Full Text Article

Enhanced HTML    Acrobat PDF (1121 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

This paper proposes a new autofocusing method for observing cells under a transmission illumination. The focusing method uses a quick and simple focus estimation technique termed “depth from diffraction,” which is based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed auto-focusing. To demonstrate the method, it was applied to continuous focus tracking of a swimming paramecium, in combination with two-dimensional position tracking. Three-dimensional tracking of the paramecium for 70 s was successfully demonstrated.

© 2006 Optical Society of America

OCIS Codes
(100.5010) Image processing : Pattern recognition
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 27, 2006
Revised Manuscript: April 12, 2006
Manuscript Accepted: April 13, 2006
Published: May 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Hiromasa Oku, Masatoshi Ishikawa, Theodorus , and Koichi Hashimoto, "High-speed autofocusing of a cell using diffraction pattern," Opt. Express 14, 3952-3960 (2006)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-14-9-3952


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. H. Price and D. A. Gough, "Comparison of Phase-Contrast and Fluorescence Digital Autofocus for Scanning Microscopy," Cytometry 16, 283-297 (1994). [CrossRef]
  2. M. Subbarao and J.-K. Tyan, "Selecting the Optimal Focus Measure for Autofocusing and Depth-From-Focus," IEEE Trans. Patternn Anal. Mach. Intell. 20, 864-870 (1998). [CrossRef]
  3. J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Greets, " Robust Autofocusing in Microscopy," Cytometry 39, 1-9 (2000). [CrossRef] [PubMed]
  4. M. Born and E. Wolf, Principles of Optics, 7th Edition (Cambridge University Press, Cambridge, 2002).
  5. W. D. Nesse, Introduction to Optical Mineralogy. (Oxford University Press, New York, 1991).
  6. T. Tsuruta. Oyo-kogaku (Applied Optics) I. (Baifukan, Tokyo, 1990). (in Japanese)
  7. H. Toyoda, N. Mukohzaka, K. Nakamura, M. Takumi, S. Mizuno, and M. Ishikawa, "1ms column-parallel vision system coupled with an image intensifier; I-CPV," in Proceedings of Symp. High Speed Photography and Photonics 2001, vol. 5-1, 2001, pp. 89-92 (in Japanese).
  8. Y. Nakabo, M. Ishikawa, H. Toyoda, and S. Mizuno, "1ms column parallel vision system and it’s application of high speed target tracking," in Proceedings of the IEEE International Conference on Robotics & Automation (Institute of Electrical and Electronics Engineers, New York, 2000), pp. 650-655.
  9. H. Oku, N. Ogawa, K. Hashimoto, and M. Ishikawa, " Two-dimensional tracking of a motile microorganism allowing high-resolution observation with various imaging techniques," Rev. Sci. Instrum. 76, 034301 (2005). [CrossRef]
  10. J. W. Goodman, Introduction to Fourier Optics. (McGraw-Hill, Inc., Boston, Massachusetts, 1996).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material


» Media 1: MOV (2507 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited