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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 13 — Jun. 25, 2007
  • pp: 7922–7932

Real-time actin-cytoskeleton depolymerization detection in a single cell using optical tweezers

Anna Chiara De Luca, Giovanni Volpe, Anna Morales Drets, Maria Isabel Geli, Giuseppe Pesce, Giulia Rusciano, Antonio Sasso, and Dmitri Petrov  »View Author Affiliations

Optics Express, Vol. 15, Issue 13, pp. 7922-7932 (2007)

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The cytoskeleton provides the backbone structure for the cellular organization, determining, in particular, the cellular mechanical properties. These are important factors in many biological processes, as, for instance, the metastatic process of malignant cells. In this paper, we demonstrate the possibility of monitoring the cytoskeleton structural transformations in optically trapped yeast cells (Saccharomyces cerevisiae) by tracking the forward scattered light via a quadrant photodiode. We distinguished normal cells from cells treated with latrunculin A, a drug which is known to induce the actin-cytoskeleton depolymerization. Since the proposed technique relies only on the inherent properties of the optical trap, without requiring external markers or biochemical sensitive spectroscopic techniques, it can be readily combined with existing optical tweezers setups.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 28, 2007
Revised Manuscript: May 11, 2007
Manuscript Accepted: June 5, 2007
Published: June 11, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Anna Chiara De Luca, Giovanni Volpe, Anna Morales Drets, Maria Isabel Geli, Giuseppe Pesce, Giulia Rusciano, Antonio Sasso, and Dmitri Petrov, "Real-time actin-cytoskeleton depolymerization detection in a single cell using optical tweezers," Opt. Express 15, 7922-7932 (2007)

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