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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 13 — Jun. 25, 2007
  • pp: 7922–7932

Real-time actin-cytoskeleton depolymerization detection in a single cell using optical tweezers

Anna Chiara De Luca, Giovanni Volpe, Anna Morales Drets, Maria Isabel Geli, Giuseppe Pesce, Giulia Rusciano, Antonio Sasso, and Dmitri Petrov  »View Author Affiliations


Optics Express, Vol. 15, Issue 13, pp. 7922-7932 (2007)
http://dx.doi.org/10.1364/OE.15.007922


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Abstract

The cytoskeleton provides the backbone structure for the cellular organization, determining, in particular, the cellular mechanical properties. These are important factors in many biological processes, as, for instance, the metastatic process of malignant cells. In this paper, we demonstrate the possibility of monitoring the cytoskeleton structural transformations in optically trapped yeast cells (Saccharomyces cerevisiae) by tracking the forward scattered light via a quadrant photodiode. We distinguished normal cells from cells treated with latrunculin A, a drug which is known to induce the actin-cytoskeleton depolymerization. Since the proposed technique relies only on the inherent properties of the optical trap, without requiring external markers or biochemical sensitive spectroscopic techniques, it can be readily combined with existing optical tweezers setups.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(140.7010) Lasers and laser optics : Laser trapping
(170.1420) Medical optics and biotechnology : Biology
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 28, 2007
Revised Manuscript: May 11, 2007
Manuscript Accepted: June 5, 2007
Published: June 11, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Anna Chiara De Luca, Giovanni Volpe, Anna Morales Drets, Maria Isabel Geli, Giuseppe Pesce, Giulia Rusciano, Antonio Sasso, and Dmitri Petrov, "Real-time actin-cytoskeleton depolymerization detection in a single cell using optical tweezers," Opt. Express 15, 7922-7932 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-13-7922


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References

  1. B. Alberts, D. Bray, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, Essential Cell Biology, (Garland, New York, 2002).
  2. K. M. Rao and H. J. Cohen, "Actin cytoskeleton network in aging and cancer," Mutat. Res. 256, 139-148 (1991). [PubMed]
  3. J. Guck, S. Schinkinger, B. Lincoln, F. Wottawah, S. Ebert, M. Romeyke, D. Lenz, H. M. Erickson, R. Ananthakrishnan, D. Mitchell, J. Kas, S. Ulvick, and C. Bilby, "Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence," Biophys. J. 88, 3689-3698 (2005). [CrossRef] [PubMed]
  4. K. A. Ward, W. I. Li, S. Zimmer and T. Davis, "Viscoelastic properties of transformed cells: role in tumor cell progression and metastasis formation," Biorheology 28, 301-313 (1991). [PubMed]
  5. R. M. Hochmuth, "Micropipette aspiration of living cells," J. Biomech. 33, 15-22 (2000). [CrossRef]
  6. I. Y. Wong, M. L. Gardel, D. R. Reichman, E. R. Weeks, M.T. Valentine, A. R. Bausch and D. A. Weitz, "Anomalous diffusion probes microstructure dynamics of entangled F-Actin natworks," Phys. Rev. Lett. 92, 178101-4 (2004). [CrossRef] [PubMed]
  7. I. M. Tolic-Norrelykke, E-L. Munteanu, G. Thon, L. Oddershede, and K. Berg-Sorensen, "Anomalous diffusion in living yeast cells," Phys. Rev. Lett. 93, 0781021-0781024 (2004). [CrossRef]
  8. G. V. Soni, B. M. J. Ali, Y. Hatwalne and G. V. Shivashankar, "Single particle tracking correlated bacterial dynamics," Biophys. J. 84, 2634-2637 (2003). [CrossRef] [PubMed]
  9. J. C. Crocker, M. T. Valentine, E. R. Weeks, T. Gisler, P. D. Kaplan, A. G. Yodh, and D. A. Weitz, "Two.point microrheology of inhomogeneous soft material," Phys. Rev. Lett. 85, 888-891 (2000). [CrossRef] [PubMed]
  10. J. Sleep, D. Wilson, R. Simmons and W. Gratzer, "Elasticy of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study," Biophys. J. 77, 3085-3095 (1999). [CrossRef] [PubMed]
  11. J. Guck, R. Ananthakrishnan, T. J. Moon, C. C. Cunningham, and J. Kas, "Optical deformability of soft biological dielectrics," Phys. Rev. Lett. 84, 5451-5454 (2000). [CrossRef] [PubMed]
  12. L. P. Ghislain and W. W. Webb, "Scanning-force microscope based on an optical trap," Opt. Lett. 18, 1678-1680 (1993). [CrossRef] [PubMed]
  13. E. L. Florin, A. Pralle, E. H. Stelzer and J. K. H. Horber, "Photonic forcemicroscope calibration by thermal noise analysis," Appl. Phys. A 66, 71-73 (1998). [CrossRef]
  14. E. Helfer, S. Harlepp, L. Bourdieu, J. Robert, F. C. MacKintosh and D. Chatenay, "Microreology of Biopolymer- Membrane Complexes," Phys. Rev. Lett. 85, 2457-460 (2000). [CrossRef] [PubMed]
  15. M. Wei, K. Yang, A. Karmenyan, and A. Chiou, "Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap," Opt. Express 14, 3056-3064 (2006). [CrossRef] [PubMed]
  16. G. Volpe, G. P. Singh, and D. Petrov, "Dynamics of a growing cell in an optical trap," Appl. Phys. Lett. 88, 2311061-3 (2006). [CrossRef]
  17. M. T. Wei and A. Chiou, "Three-dimensional tracking of Brownian motion of a particle trapped in optical tweezers with a pair of orthogonal tracking beams and the determination of the associated optical force constants," Opt. Express 13, 5798-5806 (2005). [CrossRef] [PubMed]
  18. M. Zhan, J. Renken, and S. Seeger, "Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers," FEBS Lett. 443 337-340 (1999). [CrossRef]
  19. L. Paterson, E. Papagiakoumou, G. Milne, V. Garces-Chavez, S. A. Tatarkova,W. Sibbet, F. J. Gunn-Moore, P. E. Bryant, A. Riches and K. Dholakia, "Light-induced cell separation in a tailored optical landscape," Appl. Phys. Lett. 87,123901-3 (2005). [CrossRef]
  20. J. A. Dharmadhikari, S. Roy, A. K. Dharmadhikari, S. Sharma and D. Mathur, "Torque-generating malariainfected red blood cells in an optical trapp," Opt. Express 12, 1179-1184 (2004). [CrossRef] [PubMed]
  21. S. K. Mohanty, K. S. Mohanty and P. K. Gupta, "Dynamics of interaction of rbc with optical tweezers," Opt. Express 13, 4745-4751 (2005). [CrossRef] [PubMed]
  22. K. R. Ayscough, J. Stryker, N. Pokala, M. Sanders, P. Crews, and D. G. Drubin, "High rates of actin filaments turnover in budding yeast and roles for actin in extablishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin A," J. Cell. Biol. 137, 399-416 (1997). [CrossRef] [PubMed]
  23. M. Cou, S. L. Brenner, I. Spector and E. D. Korn, "Inhibition of actin polymerization by latrunculin A," FEBS Lett. 213, 316-318 (1987). [CrossRef]
  24. S. C. Kuo, "A single assay for local heating by optical tweezers," Methods Cell Biol. 55, 43-45 (1998). [CrossRef]
  25. E. J. G. Peterman, F. Gittes and C. F. Schmidt, "Laser-induced heating in optical traps," Biophys. J. 84, 1308- 1316 (2003). [CrossRef] [PubMed]
  26. G. P. Singh, G. Volpe, C. M. Creely, H. Grotsch, I. M. Geli, and D. Petrov, "The lag phase and G1 phase of a single yeast cell monitored by Raman microspectroscopy," J. Raman Spectrosc. 37, 858-864 (2006). [CrossRef]
  27. C. F. Robinow and J. S. Hyams, in Molecular biology of the fission yeast, A. Nasim, P. Young, and B. F. Jonson, eds. (Academic Press, Inc. San Diego, 1989), pp. 273-330.
  28. T. A. Vida and S. D. Emr, "A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast," J. Cell Biol. 128, 779-792 (1995). [CrossRef] [PubMed]
  29. J. Achilles, H. Harms and S. Muller, "Analysis of living S. cerevisiae cell states-A three color approach," Cytometry A. 69, 173-177 (2006). [CrossRef]
  30. L. Sacconi, I. M. Tolic-Nørrelykke, C. Stringari, R. Antolini and F. S. Pavone, "Optical micromanipulations inside yeast cells," Appl. Opt. 44, 2001-2007 (2005). [CrossRef] [PubMed]
  31. K. L. Hill, N. L Catlett, and L. S. Weisman, "Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae," J. Cell Biol. 135, 1535-1549 (1996). [CrossRef] [PubMed]
  32. K. L. Fehrenbacher, H. C. Yang, A. C. Gay, T. M. Huckaba, and L. A. Pon, "Live cell imaging of mitochondrial movement along actin cables in budding yeast," Curr. Biol. 14,1996-2004 (2004). [CrossRef] [PubMed]
  33. K. M. Addas, C. F. Schmidt, and J. X. Tang, "Microrheology of solutions of semiflexible biopolymer filaments using laser tweezers interferometry," Phys. Rev. E 70, 021503 1-16 (2004). [CrossRef]

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