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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 19 — Sep. 17, 2007
  • pp: 11874–11882

Development of a handheld Raman microspectrometer for clinical dermatologic applications

Chad A. Lieber and Anita Mahadevan-Jansen  »View Author Affiliations

Optics Express, Vol. 15, Issue 19, pp. 11874-11882 (2007)

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Although skin is easily accessible to optical methodologies, a portable measurement head is necessary to allow ready spectroscopic interrogation of all anatomic locations. However, most conventional Raman microspectrometers and even dermatologic-specific Raman systems are fixed systems ill-suited to anatomic accessibility. To this end, we have developed a portable Raman microspectrometer system for future dermatologic studies. An in-house-built bench-top system was used to qualify the optical components and design. Based on this system’s layout, a handheld microspectrometer was developed for future clinical application. This system produces similar operating characteristics to the bench-top prototype, and is shown to provide clear Raman spectra from skin tissue measured in vivo in clinically-feasible integration times.

© 2007 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1870) Medical optics and biotechnology : Dermatology
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(300.6450) Spectroscopy : Spectroscopy, Raman

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 11, 2007
Revised Manuscript: August 16, 2007
Manuscript Accepted: August 25, 2007
Published: September 4, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Chad Lieber and Anita Mahadevan-Jansen, "Development of a handheld Raman microspectrometer for clinical dermatologic applications," Opt. Express 15, 11874-11882 (2007)

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  1. Y. E. Gorbaty and G. V. Bondarenko, "High-pressure high-temperature Raman cell for corrosive liquids," Rev. Sci. Instrum. 66, 4347-4349 (1995). [CrossRef]
  2. D. Schiferl, S. K. Sharma, T. F. Cooney, S. Y. Wang, and K. Mohanan, "Multichannel Raman spectrometry system for weakly scattering materials at simultaneous high pressures and high temperatures," Rev. Sci. Instrum. 64, 2821-2827 (1993). [CrossRef]
  3. D. L. Dickensheets, D. D. Wynn-Williams, H. G. M. Edwards, C. Schoen, C. Crowder, and E. M. Newton, "A novel miniature confocal microscope/Raman spectrometer system for biomolecular analysis on future Mars missions after Antarctic trials," J. Raman Spectrosc. 31, 633-635 (2000). [CrossRef]
  4. H. G. Edwards, E. M. Newton, D. L. Dickensheets, and D. D. Wynn-Williams, "Raman spectroscopic detection of biomolecular markers from Antarctic materials: evaluation for putative Martian habitats," Spectrochim. Acta A 59, 2277-2290 (2003). [CrossRef]
  5. A. Wang, L. A. Haskin, and E. Cortez, "Prototype Raman spectroscopic sensor for in situ mineral characterization on planetary surfaces," Appl. Spectrosc. 52, 477-487 (1998). [CrossRef]
  6. E. B. Hanlon, R. Manoharan, T. W. Koo, K. E. Shafer, J. T. Motz, M. Fitzmaurice, J. R. Kramer, I. Itzkan, R. R. Dasari, and M. S. Feld, "Prospects for in vivo Raman spectroscopy," Phys. Med. Biol. 45, R1-59 (2000). [CrossRef] [PubMed]
  7. A. Mahadevan-Jansen, and R. Richards-Kortum, "Raman spectroscopy for the detection of cancers and precancers," J. Biomed. Opt. 1, 31-70 (1996). [CrossRef]
  8. N. Stone, C. Kendall, N. Shepherd, P. Crow, and H. Barr, "Near-infrared Raman spectroscopy for the classification of epithelial pre-cancers and cancers," J. Raman Spectrosc. 33, 564-573 (2002). [CrossRef]
  9. P. J. Caspers, G. W. Lucassen, E. A. Carter, H. A. Bruining, and G. J. Puppels, "In vivo confocal Raman microspectroscopy of the skin: Noninvasive determination of molecular concentration profiles," J. Invest. Dermatol. 116, 434-442 (2001). [CrossRef] [PubMed]
  10. L. Chrit, P. Bastien, G. D. Sockalingum, D. Batisse, F. Leroy, M. Manfait, and C. Hadjur, "An in vivo randomized study of human skin moisturization by a new confocal Raman fiber-optic microprobe: assessment of a glycerol-based hydration cream," Skin pharmacology and physiology 19, 207-215 (2006). [CrossRef] [PubMed]
  11. H. P. Buschman, G. Deinum, J. T. Motz, M. Fitzmaurice, J. R. Kramer, A. van der Laarse, A. V. Bruschke, and M. S. Feld, "Raman microspectroscopy of human coronary atherosclerosis: Biochemical assessment of cellular and extracellular morphologic structures in situ," Cardiovasc. Pathol. 10, 69-82 (2001). [CrossRef] [PubMed]
  12. H. P. Buschman, E. T. Marple, M. L. Wach, B. Bennett, T. C. Schut, H. A. Bruining, A. V. Bruschke, A. van der Laarse, and G. J. Puppels, "In vivo determination of the molecular composition of artery wall by intravascular Raman spectroscopy," Anal. Chem. 72, 3771-3775 (2000). [CrossRef] [PubMed]
  13. R. Erckens, M. Motamedi, and W. March, "Raman spectroscopy for non-invasive characterization of ocular tissue: potential for detection of biological molecules," J. Raman Spectrosc. 28, 293-299 (1997). [CrossRef]
  14. P. J. Caspers, G. W. Lucassen, H. A. Bruining, and G. J. Puppels, "Automated depth-scanning confocal Raman microspectrometer for rapid in vivo determination of water concentration profiles in human skin," J. Raman Spectrosc. 31, 813-818 (2000). [CrossRef]
  15. L. Chrit, C. Hadjur, S. Morel, G. Sockalingum, G. Lebourdon, F. Leroy, and M. Manfait, "In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe," J. Biomed. Opt. 10, 44007 (2005). [CrossRef] [PubMed]
  16. A. Nijssen, T. C. Bakker Schut, F. Heule, P. J. Caspers, D. P. Hayes, M. H. A. Neumann, and G. J. Puppels, "Discriminating basal cell carcinoma from its surrounding tissue by Raman spectroscopy," J. Invest. Dermatol. 119, 64-69 (2002). [CrossRef] [PubMed]
  17. P. J. Caspers, G. W. Lucassen, and G. J. Puppels, "Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin," Biophys. J. 85, 572-580 (2003). [CrossRef] [PubMed]
  18. M. Rajadhyaksha, S. Gonzalez, J. M. Zavislan, R. R. Anderson, and R. H. Webb, "In vivo confocal scanning laser microscopy of human skin II: Advances in instrumentation and comparison with histology," J. Invest. Dermatol. 113, 293-303 (1999). [CrossRef] [PubMed]
  19. M. Rajadhyaksha, M. Grossman, D. Esterowitz, R. H. Webb, and R. R. Anderson, "In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast," J. Invest. Dermatol. 104, 946-952 (1995). [CrossRef] [PubMed]
  20. L. D. Swindle, S. G. Thomas, M. Freeman, and P. M. Delaney, "View of normal human skin in vivo as observed using fluorescent fiber-optic confocal microscopic imaging," J. Invest. Dermatol. 121, 706-712 (2003). [CrossRef] [PubMed]
  21. J. P. Kelly, A. H. Weiss, Q. Zhou, S. Schmode, and A. W. Dreher, "Imaging a child's fundus without dilation using a handheld confocal scanning laser ophthalmoscope," Arch. Ophthalmol. (Chicago). 121, 391-396 (2003).
  22. R. Tabaksblat, R. J. Meier, and B. J. Kip, "Confocal Raman microspectroscopy: Theory and application to thin polymer samples," Appl. Spectrosc. 46, 60-68 (1992). [CrossRef]
  23. T. Wilson, and A. R. Carlini, "Size of the detector in confocal imaging systems," Opt. Lett. 12, 227-229 (1987). [CrossRef] [PubMed]

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