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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 19 — Sep. 17, 2007
  • pp: 12050–12059

Differential imaging in coherent anti-Stokes Raman scattering microscopy II: a filter-assisted Laguerre-Gaussian beam detection scheme

Liu Cheng, Suhas P. Veetil, and Dug Young Kim  »View Author Affiliations

Optics Express, Vol. 15, Issue 19, pp. 12050-12059 (2007)

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A new differential imaging technique to obtain contrast improvement in coherent anti-Stokes Raman scattering (CARS) microscopy is proposed through a spatial spiral phase modulation in the collected signal of CARS microscopy. A spiral phase mask makes the CARS signal from the bulk material to be distributed in a ring centered at the detecting pinhole of a confocal microscope resulting in a weak detected CARS signal from a bulk material. When tiny scatters are included in the focal volume of a CARS setup, the ring-shaped distribution of CARS field is distorted, leading to an increase in the detected signal through the pinhole. The sensitivity and the size selectivity of this proposed technique is studied with varying the particle size, and it is found that this method is to be efficient in edge detection. Simulation results obtained by finite difference time domain (FDTD) methods show that the image contrast is enhanced by many times as it is able to highlight the details of the specimen by suppressing the CARS signal from a bulk or a uniform material.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(290.5860) Scattering : Scattering, Raman
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(330.6130) Vision, color, and visual optics : Spatial resolution

ToC Category:

Original Manuscript: August 1, 2007
Revised Manuscript: August 30, 2007
Manuscript Accepted: September 2, 2007
Published: September 6, 2007

Virtual Issues
Vol. 2, Iss. 10 Virtual Journal for Biomedical Optics

Liu Cheng, Suhas Veetil, and DugYoung Kim, "Differential imaging in coherent anti-Stokes Raman scattering microscopy II: a filter-assisted Laguerre-Gaussian beam detection scheme," Opt. Express 15, 12050-12059 (2007)

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