OSA's Digital Library

Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 20 — Oct. 1, 2007
  • pp: 12662–12679

Proposal of three-dimensional phase contrast holographic microscopy

Naoki Fukutake and Tom D. Milster  »View Author Affiliations

Optics Express, Vol. 15, Issue 20, pp. 12662-12679 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (543 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We propose a three-dimensional phase contrast digital holographic microscopy. The object to be observed is a low-contrast transparent refractive index distribution sample, such as biological tissue. Low contrast phase objects are converted to high contrast images through the microscopy we propose. In order to gain high three-dimensional resolution, the direction of pump plane wave is scanned, and separate holographic images produced at each angle are acquired and decoded into complex amplitude in Fourier space. The three-dimensional image is reconstructed in a computer from all information acquired through the system. The resolution in the direction of the optical axis is increased by utilizing a 4π configuration of objective lenzes.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: August 10, 2007
Revised Manuscript: September 11, 2007
Manuscript Accepted: September 13, 2007
Published: September 18, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Naoki Fukutake and Tom D. Milster, "Proposal of three-dimensional phase contrast holographic microscopy," Opt. Express 15, 12662-12679 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. Wilson, Confocal Microscopy (Academic Press, 1990).
  2. W. B. Amos, J. G. White, and M. Fordham, "Use of confocal imaging in the study of biological structures," Appl. Opt. 26, 3239 (1987). [CrossRef] [PubMed]
  3. G. J. Brakenhoff, H. T. M. van der Voort, E. A. van Spronsen, and N. Nanninga, "3-Dimensional imaging of biological structures by high resolution confocal scanning laser microscopy," Scanning Microsc. 2, 33 (1988). [PubMed]
  4. I. Freund and M. Deutsch, "2nd-harmonic microscopy of biological tissue," Opt. Lett. 11, 94 (1986). [CrossRef] [PubMed]
  5. P. J. Campagnola, H. A. Clark, W. A. Mohler, A. Lewis, and L. M. Loew, "Second -harmonic imaging microscopy of living cells," J. Biomed. Opt. 6, 277 (2001). [CrossRef] [PubMed]
  6. J. Mertz and L. Moreaux, "Second -harmonic generation by focused excitation of inhomogeneously distributed scatterers," Opt. Commun. 196, 325 (2001). [CrossRef]
  7. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third-harmonic generation," Appl. Phys. Lett. 70, 922 (1997). [CrossRef]
  8. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D microscopy of transparent objects using third-harmonic generation," J. Microsc. 191, 266 (1998). [CrossRef] [PubMed]
  9. M. D. Duncan, J. Reintjes, and T. J. Manuccia, "Scanning coherent anti-Stokes Raman microscope," Opt. Lett. 7, 350 (1982). [CrossRef] [PubMed]
  10. A. Zumbusch, G. R. Holtom, and X. S. Xie, "Vibrational microscopy using coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82, 4014 (1999).
  11. F. Zernike, "Das Phasenkontrastverfahren bei der mikroskopischen Beobachtung," Z. Tech. Phys. 16, 454 (1935).
  12. F. Zernike, "How I discovered phase contrast," Science 121, 345 (1955). [CrossRef] [PubMed]
  13. W. S. Haddad, D. Cullen, J. C. Solem, J. W. Longworth, A. McPherson, K. Boyer, and C. K. Rhodes, "Fourier-transform holographic microscope," Appl. Opt. 31, 4973 (1992). [CrossRef] [PubMed]
  14. U. Schnars and W. Jüptner, "Direct recording of holograms by a CCD target and numerical reconstruction," Appl. Opt. 33, 179 (1994). [CrossRef] [PubMed]
  15. J. H. Massig, "Digital off-axis holography with a synthetic aperture," Opt. Lett. 27, 2179 (2002). [CrossRef]
  16. S. Kostianovski, S. G. Lipson, and E. N. Ribak, "Interference microscopy and Fourier fringe analysis applied to measuring the spatial refractive-index distribution," Appl. Opt. 32,4744 (1993). [CrossRef] [PubMed]
  17. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, "Optical coherence tomography," Science  254, 1178 (1991). [CrossRef] [PubMed]
  18. T. Dresel, G. Hausler, and H. Venzke, "Three-dimensional sensing of rough surfaces by coherence radar," Appl. Opt. 31, 919 (1992). [CrossRef] [PubMed]
  19. M. Mansuripur, Classical Optics and its Applications (Cambridge University Press 2002)
  20. M. Born and E. Wolf, Principles of Optics 5th. ed., (Pergamon Press, 1974).
  21. H. Kogelnik, "Coupled wave theory for thick hologram gratings," Bell Syst. Tech. J. 48, 2909 (1969).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited