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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 22 — Oct. 29, 2007
  • pp: 14591–14600

Quantitative Phase Microscopy of microstructures with extended measurement range and correction of chromatic aberrations by multiwavelength digital holography

P. Ferraro, L. Miccio, S. Grilli, M. Paturzo, S. De Nicola, A. Finizio, R. Osellame, and P. Laporta  »View Author Affiliations

Optics Express, Vol. 15, Issue 22, pp. 14591-14600 (2007)

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Quantitative Phase Microscopy (QPM) by interferometric techniques can require a multiwavelength configuration to remove 2p ambiguity and improve accuracy. However, severe chromatic aberration can affect the resulting phase-contrast map. By means of classical interference microscope configuration it is quite unpractical to correct such aberration. We propose and demonstrate that by Digital Holography (DH) in a microscope configuration it is possible to clear out the QPM map from the chromatic aberration in a simpler and more effective way with respect to other approaches. The proposed method takes benefit of the unique feature of DH to record in a plane out-of-focus and subsequently reconstruct numerically at the right focal image plane. In fact, the main effect of the chromatic aberration is to shift differently the correct focal image plane at each wavelength and this can be readily compensated by adjusting the corresponding reconstruction distance for each wavelength. A procedure is described in order to determine easily the relative focal shift among different imaging wavelengths by performing a scanning of the numerical reconstruction along the optical axis, to find out the focus and to remove at the same time the chromatic aberration.

© 2007 Optical Society of America

OCIS Codes
(070.4560) Fourier optics and signal processing : Data processing by optical means
(090.1000) Holography : Aberration compensation
(090.2880) Holography : Holographic interferometry

ToC Category:

Original Manuscript: July 23, 2007
Revised Manuscript: September 7, 2007
Manuscript Accepted: September 12, 2007
Published: October 19, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

P. Ferraro, L. Miccio, S. Grilli, M. Paturzo, S. De Nicola, A. Finizio, R. Osellame, and P. Laporta, "Quantitative Phase Microscopy of microstructures with extended measurement range and correction of chromatic aberrations by multiwavelength digital holography," Opt. Express 15, 14591-14600 (2007)

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