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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 23 — Nov. 12, 2007
  • pp: 15101–15114

Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation

P. T. Goedhart, M. Khalilzada, R. Bezemer, J. Merza, and C. Ince  »View Author Affiliations


Optics Express, Vol. 15, Issue 23, pp. 15101-15114 (2007)
http://dx.doi.org/10.1364/OE.15.015101


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Abstract

Sidestream Dark Field (SDF) imaging, a stroboscopic LED ring-based imaging modality, is introduced for clinical observation of the microcirculation. SDF imaging is validated by comparison to Orthogonal Polarization Spectral imaging. Nailfold capillary diameters and red blood cell velocities were measured using both techniques and equal quantitative results were obtained. An image quality system was developed to quantitatively compare the quality of sublingually-acquired microcirculatory images using OPS and SDF imaging. Venular contrast, sharpness, and quality were shown to be comparable for OPS and SDF imaging. However, capillary contrast and quality were shown to be significantly higher using SDF imaging. Venular granularity, in addition, was more clearly observable using SDF imaging.

© 2007 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.0110) Medical optics and biotechnology : Imaging systems
(170.5380) Medical optics and biotechnology : Physiology

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 7, 2007
Revised Manuscript: October 11, 2007
Manuscript Accepted: October 25, 2007
Published: October 31, 2007

Virtual Issues
Vol. 2, Iss. 12 Virtual Journal for Biomedical Optics

Citation
P. T. Goedhart, M. Khalilzada, R. Bezemer, J. Merza, and C. Ince, "Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation," Opt. Express 15, 15101-15114 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-23-15101


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