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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 25 — Dec. 10, 2007
  • pp: 16839–16851

Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence

Sonny Ly, Gregory McNerney, Samantha Fore, James Chan, and Thomas Huser  »View Author Affiliations

Optics Express, Vol. 15, Issue 25, pp. 16839-16851 (2007)

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We demonstrate time-gated confocal imaging as a means to separate coherent anti-Stokes Raman scattering (CARS) microscopy data from multi-photon excited endogenous fluorescence in tissue. CARS is a quasi-instantaneous process and its signal decay time is only limited by the system’s instrument response function (IRF). Signals due to two-photon-excited (TPE) tissue autofluorescence with excited state lifetimes on the nanosecond scale can be identified and separated from the CARS signal by employing time-gating techniques. We demonstrate this improved contrast on the example of CARS microscopy of intact roots of plant seedlings as well as on rat arterial tissue.

© 2007 Optical Society of America

OCIS Codes
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(190.5650) Nonlinear optics : Raman effect
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 2, 2007
Revised Manuscript: November 30, 2007
Manuscript Accepted: December 3, 2007
Published: December 4, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Sonny Ly, Gregory McNerney, Samantha Fore, James Chan, and Thomas Huser, "Time-gated single photon counting enables separation of CARS microscopy data from multiphoton-excited tissue autofluorescence," Opt. Express 15, 16839-16851 (2007)

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