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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 3 — Feb. 5, 2007
  • pp: 1043–1053

Intrinsic fluorescence changes associated with the conformational state of silk fibroin in biomaterial matrices

Irene Georgakoudi, Irene Tsai, Cherry Greiner, Cheryl Wong, Jordy DeFelice, and David Kaplan  »View Author Affiliations


Optics Express, Vol. 15, Issue 3, pp. 1043-1053 (2007)
http://dx.doi.org/10.1364/OE.15.001043


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Abstract

Silk fibroin is emerging as an important biomaterial for tissue engineering applications. The ability to monitor non-invasively the structural conformation of silk matrices prior to and following cell seeding could provide important insights with regards to matrix remodeling and cell-matrix interactions that are critical for the functional development of silk-based engineered tissues. Thus, we examined the potential of intrinsic fluorescence as a tool for assessing the structural conformation of silk proteins. Specifically, we characterized the intrinsic fluorescence spectra of silk in solution, gel and scaffold configurations for excitation in the 250 to 335 nm range and emission from 265 to 600 nm. We have identified spectral components that are attributed to tyrosine, tryptophan and crosslinks based on their excitation-emission profiles. We have discovered significant spectral shifts in the emission profiles and relative contributions of these components among the silk solution, gel and scaffold samples that represent enhancements in the levels of crosslinking, hydrophobic and intermolecular interactions that are consistent with an increase in the levels of ß-sheet formation and stacking. This information can be easily utilized for the development of simple, non-invasive, ratiometric methods to assess and monitor the structural conformation of silk in engineered tissues.

© 2007 Optical Society of America

OCIS Codes
(170.1580) Medical optics and biotechnology : Chemometrics
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 16, 2007
Manuscript Accepted: January 29, 2007
Published: February 5, 2007

Virtual Issues
Vol. 2, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Irene Georgakoudi, Irene Tsai, Cherry Greiner, Cheryl Wong, Jordy DeFelice, and David Kaplan, "Intrinsic fluorescence changes associated with the conformational state of silk fibroin in biomaterial matrices," Opt. Express 15, 1043-1053 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-3-1043


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