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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 15, Iss. 8 — Apr. 16, 2007
  • pp: 4390–4397

Competitive displacement: A sensitive and selective method for the detection of unlabeled molecules

J. Bishop, A. M. Chagovetz, and S. Blair  »View Author Affiliations


Optics Express, Vol. 15, Issue 8, pp. 4390-4397 (2007)
http://dx.doi.org/10.1364/OE.15.004390


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Abstract

We propose a new method for molecular detection that retains the sensitivity of fluorescence, but without requiring fluorescence labeling of the sample. The method works by spiking the sample solution with one or more labeled molecular species of known concentration. With proper choice of these “competitor” species, their binding kinetics can be used to quantitatively determine the concentration of unlabeled target species. This method can be applied to any fluorescence transduction mechanism that allows real-time signal acquisition, and represents an advance in mitigating certain sample processing steps. We demonstrate the method for the detection of a DNA sequence containing a single-nucleotide polymorphism (SNP).

© 2007 Optical Society of America

OCIS Codes
(130.6010) Integrated optics : Sensors
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Detectors

History
Original Manuscript: January 8, 2007
Revised Manuscript: March 17, 2007
Manuscript Accepted: March 29, 2007
Published: April 3, 2007

Virtual Issues
Vol. 2, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Justin Bishop, Alexander M. Chagovetz, and Steve Blair, "Competitive displacement: A sensitive and selective method for the detection of unlabeled molecules," Opt. Express 15, 4390-4397 (2007)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-15-8-4390


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