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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 12 — Jun. 9, 2008
  • pp: 8519–8531

Detection efficiency in total internal reflection fluorescence microscopy

Marcel Leutenegger and Theo Lasser  »View Author Affiliations

Optics Express, Vol. 16, Issue 12, pp. 8519-8531 (2008)

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We present a rapid and flexible framework for the accurate calculation of the detection efficiency of fluorescence emission in isotropic media as well as in the vicinity of dielectric or metallic interfaces. The framework accounts for the dipole characteristics of the emitted fluorescence and yields the absolute detection efficiency by taking into account the total power radiated by the fluorophore. This analysis proved to be useful for quantitative measurements, i.e. the fluorescence detection at a glass–water interface for total internal reflection fluorescence microscopy in an epi- and a trans-illumination configuration.

© 2008 Optical Society of America

OCIS Codes
(110.2990) Imaging systems : Image formation theory
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(240.6490) Optics at surfaces : Spectroscopy, surface

ToC Category:

Original Manuscript: March 28, 2008
Revised Manuscript: May 9, 2008
Manuscript Accepted: May 23, 2008
Published: May 27, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Marcel Leutenegger and Theo Lasser, "Detection efficiency in total internal reflection fluorescence microscopy," Opt. Express 16, 8519-8531 (2008)

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