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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 12 — Jun. 9, 2008
  • pp: 9011–9020

Multiple holographic optical tweezers parallel calibration with optical potential well characterization

Federico Belloni, Serge Monneret, Fabien Monduc, and Maxime Scordia  »View Author Affiliations

Optics Express, Vol. 16, Issue 12, pp. 9011-9020 (2008)

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We report the extension to a multi-axes exploration of the potential well reconstruction method against drag force to simultaneously characterize the potential wells of several trapping sites generated with holographic optical tweezers. The final result is a robust, fast and automatic procedure we use to characterize holographic tweezers. We mainly focus on the reliability of the method and its application to address the dependence of the diffraction efficiency with the trap position in a given holographic traps pattern.

© 2008 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: November 14, 2007
Revised Manuscript: December 13, 2007
Manuscript Accepted: January 24, 2008
Published: June 4, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Federico Belloni, Serge Monneret, Fabien Monduc, and Maxime Scordia, "Multiple holographic optical tweezers parallel calibration with optical potential well characterization," Opt. Express 16, 9011-9020 (2008)

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  1. P. N. Lebedev, "Experimental examination of light pressure," Annalen der Physik 6, 433 (1901).
  2. A. Ashkin, J. M. Dziedzic, J. E. Bjorkholm, and S. Chu, "Observation of a single-beam gradient force optical trap for dielectric particles," Opt. Lett. 11, 288-290 (1986). [CrossRef] [PubMed]
  3. G. Lenormand, S. Henon, A. Richert, J. Simeon, and F. Gallet, "Direct measurement of the area expansion and shear moduli of the human red blood cell membrane skeleton," Biophys. J. 81, 43-56 (2001). [CrossRef] [PubMed]
  4. K. Svoboda and S. M. Block, "Force and velocity measured for single kinesin molecules," Cell 77, 773-784 (1994). [CrossRef] [PubMed]
  5. R. M. Simmons, J. T. Finer, S. Chu, and J. A. Spudich, "Quantitative measurements of force and displacement using an optical trap," Biophys. J. 70, 1813-1822 (1996). [CrossRef] [PubMed]
  6. J. C. Meiners and S. R. Quake, "Femtonewton force spectroscopy of single extended dna molecules," Phys. Rev. Lett. 84, 5014-5017 (2000). [CrossRef] [PubMed]
  7. M. Polin, K. Ladavac, S.-H. Lee, Y. Roichman, and D. Grier, "Optimized holographic optical traps," Opt. Express 13, 5831-5845 (2005) [CrossRef] [PubMed]
  8. N. Malagnino, G. Pesce, A. Sasso, and E. Arimondo, "Measurements of trapping efficiency and stiffness in optical tweezers," Opt. Commun. 214, 15-24 (2002). [CrossRef]
  9. E. R. Dufresne and D. G. Grier, "Optical tweezer arrays and optical substrates created with diffractive optical elements," Rev. Sci. Instr. 69, 1974-1977 (1998). [CrossRef]
  10. D. G. Grier, "A revolution in optical manipulation," Nature (London) 424, 810-816 (2003). [CrossRef] [PubMed]
  11. A. Rohrbach and E. H. K. Stelzer, "Trapping forces, force constants, and potential depths for dielectric spheres in the presence of spherical aberrations," Appl. Opt. 41, 2494-2507 (2002). [CrossRef] [PubMed]
  12. M. Capitanio, G. Romano, R. Ballerini, M. Giuntini, F. S. Pavone, D. Dunlap, and L. Finzi, "Calibration of optical tweezers with differential interference contrast signals," Rev. Sci. Instrum. 73, 1687-1696 (2002). [CrossRef]
  13. K. Berg-Sorensen and H. Flyvbjerg, "Power spectrum analysis for optical tweezers," Rev. Sci. Instrum. 75, 594-612 (2004). [CrossRef]
  14. M. Klein, M. Andersson, O. Axner, and E. Fallman, "Dual-trap technique for reduction of low-frequency noise in force measuring optical tweezers," Appl. Opt. 46, 405-412 (2007). [CrossRef] [PubMed]
  15. H. Faxen, "Die Bewegung einer starren Kugel langs der Achse eines mit zahrer Flbsigkeit gefiillten Rohres," Ark. Mat. Astron. Fys. 17, 1-28 (1923).
  16. S. Keen, J. Leach, G. Gibson, and M. J. Padgett, "Comparison of a high-speed camera and a quadrant detector for measuring displacements in optical tweezers," J. Opt. A Pure Appl. Opt. 9, S264-S266 (2007). [CrossRef]
  17. W. Singer, S. Bernet, N. Hecker, and M. Ritsch-Marte, "Three-dimensional force calibration of optical tweezers," J. Mod. Opt. 47, 2921-2931 (2000).
  18. Y. K. Nahmias, B. Zhi Gao, and D. J. Odde, "Dimensionless parameters for the design of optical traps and laser guidance Ssstems," Appl. Opt. 43, 3999-4006 (2004). [CrossRef] [PubMed]
  19. F. Belloni and S. Monneret, "Quadrant kinoform: an approach to multiplane dynamic three-dimensional holographic trapping," Appl. Opt. 46, 4587-4593 (2007). [CrossRef] [PubMed]
  20. S. Monneret, F. Belloni, and O. Soppera. "Combining fluidic reservoirs and optical tweezers to control beads/living cells contacts," Microfluid. Nanofluid. (2007). [CrossRef]

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