Adaptive optics for structured illumination microscopy

doi:10.1364/OE.16.009290

Adaptive optics for structured illumination microscopy

Delphine Débarre, Edward J. Botcherby, Martin J. Booth, and Tony Wilson »View Author Affiliations

Optics Express, Vol. 16, Issue 13, pp. 9290-9305 (2008)
http://dx.doi.org/10.1364/OE.16.009290

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Abstract

We implement wave front sensor-less adaptive optics in a structured illumination microscope. We investigate how the image formation process in this type of microscope is affected by aberrations. It is found that aberrations can be classified into two groups, those that affect imaging of the illumination pattern and those that have no influence on this pattern. We derive a set of aberration modes ideally suited to this application and use these modes as the basis for an efficient aberration correction scheme. Each mode is corrected independently through the sequential optimisation of an image quality metric. Aberration corrected imaging is demonstrated using fixed fluorescent specimens. Images are further improved using differential aberration imaging for reduction of background fluorescence.

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Adaptive Optics

History
Original Manuscript: April 11, 2008
Revised Manuscript: May 30, 2008
Manuscript Accepted: June 3, 2008
Published: June 9, 2008

Virtual Issues
Vol. 3, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Delphine Débarre, Edward J. Botcherby, Martin J. Booth, and Tony Wilson, "Adaptive optics for structured illumination microscopy," Opt. Express 16, 9290-9305 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-13-9290

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References

J. B. Pawley, ed., Handbook of Biological Confocal Microscopy, 3rd Edition (Springer, New York, 2006). [CrossRef]
J. A. Conchello and J. W. Lichtman, "Optical sectioning microscopy," Nature Methods 2(12), 920-931 (2005). [CrossRef] [PubMed]
M. A. A. Neil, R. Juškaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997). [CrossRef]
M. J. Booth, "Adaptive optics in microscopy," Philos. Transact. A Math. Phys. Eng. Sci. 365, 2829-2843 (2007). [CrossRef] [PubMed]
M. J. Booth, M. A. A. Neil, R. Juškaitis, and T. Wilson, "Adaptive aberration correction in a confocal microscope," Proc. Nat. Acad. Sci. 99, 5788-5792 (2002). [CrossRef] [PubMed]
D. Débarre, M. J. Booth, and T. Wilson, "Image based adaptive optics through optimisation of low spatial frequencies," Opt. Express 15, 8176-8190 (2007). [CrossRef] [PubMed]
H. Hopkins, "The use of diffraction-based criteria of image quality in automatic optical design," Opt. Acta 13, 343-69 (1966). [CrossRef]
D. Karadagli?? and T. Wilson, "Image formation in structured illumination wide-field fluorescence microscopy," Micron (2008, in press). [CrossRef] [PubMed]
E. W. Weisstein, CRC Concise Encyclopedia of Mathematics (Chapman and Hall/CRC, 2003).
M. A. A. Neil, M. J. Booth, and T. Wilson, "New modal wavefront sensor: a theoretical analysis," J. Opt. Soc. Am. A 17, 1098-1107, (2000). [CrossRef]
M. J. Booth, T. Wilson, H.-B. Sun, T. Ota, and S. Kawata, "Methods for the characterisation of deformable membrane mirrors," Appl. Opt. 44(24), 5131-5139 (2005). [CrossRef] [PubMed]
A. Leray and J. Mertz, "Rejection of two-photon fluorescence background in thick tissue by differential aberration imaging," Opt. Express 14, 10,565-10,573 (2006). [CrossRef]

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