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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 15 — Jul. 21, 2008
  • pp: 10900–10911

Phase imaging of cells by simultaneous dual-wavelength reflection digital holography

Alexander Khmaladze, Myung Kim, and Chun-Min Lo  »View Author Affiliations


Optics Express, Vol. 16, Issue 15, pp. 10900-10911 (2008)
http://dx.doi.org/10.1364/OE.16.010900


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Abstract

We present a phase-imaging technique to quantitatively study the three-dimensional structure of cells. The method, based on the simultaneous dual-wavelength digital holography, allows for higher axial range at which the unambiguous phase imaging can be performed. The technique is capable of nanometer axial resolution. The noise level, which increases as a result of using two wavelengths, is then reduced to the level of a single wavelength. The method compares favorably to software unwrapping, as the technique does not produce non-existent phase steps. Curvature mismatch between the reference and object beams is numerically compensated. The 3D images of SKOV-3 ovarian cancer cells are presented.

© 2008 Optical Society of America

OCIS Codes
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: April 29, 2008
Revised Manuscript: June 27, 2008
Manuscript Accepted: June 30, 2008
Published: July 7, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Alexander Khmaladze, Myung Kim, and Chun-Min Lo, "Phase imaging of cells by simultaneous dual-wavelength reflection digital holography," Opt. Express 16, 10900-10911 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-15-10900


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