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Optics Express

Optics Express

  • Vol. 16, Iss. 16 — Aug. 4, 2008
  • pp: 12227–12238

Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology

Toyohiko Yamauchi, Hidenao Iwai, Mitsuharu Miwa, and Yutaka Yamashita  »View Author Affiliations

Optics Express, Vol. 16, Issue 16, pp. 12227-12238 (2008)

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We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.

© 2008 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(110.3175) Imaging systems : Interferometric imaging

ToC Category:

Original Manuscript: May 14, 2008
Revised Manuscript: July 1, 2008
Manuscript Accepted: July 28, 2008
Published: July 31, 2008

Virtual Issues
Vol. 3, Iss. 9 Virtual Journal for Biomedical Optics

Toyohiko Yamauchi, Hidenao Iwai, Mitsuharu Miwa, and Yutaka Yamashita, "Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology," Opt. Express 16, 12227-12238 (2008)

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