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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 17 — Aug. 18, 2008
  • pp: 12819–12834

Rapid simulation of wide-angle scattering from mitochondria in single cells

Patrick M. Pilarski, Xuan-Tao Su, D. Moira Glerum, and Christopher J. Backhouse  »View Author Affiliations


Optics Express, Vol. 16, Issue 17, pp. 12819-12834 (2008)
http://dx.doi.org/10.1364/OE.16.012819


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Abstract

It has been shown that the mitochondria are the dominant source of large-angle light scattering from human cells. In the limit of small mitochondria, we show that the large-angle (isotropic) light scattering of mitochondria may be analyzed and simulated with an adaptation of classical X-ray diffraction theory. In addition, we show that this approach may be extended to the case of anisotropic scatter. These results enable the rapid simulation and analysis of mitochondrial scattering patterns and allow the determination of some aspects of cell structure directly from experimental scattering patterns.

© 2008 Optical Society of America

OCIS Codes
(000.1430) General : Biology and medicine
(100.2960) Image processing : Image analysis
(100.3190) Image processing : Inverse problems
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(290.3200) Scattering : Inverse scattering

ToC Category:
Scattering

History
Original Manuscript: March 11, 2008
Revised Manuscript: July 9, 2008
Manuscript Accepted: July 10, 2008
Published: August 8, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Patrick M. Pilarski, Xuan-Tao Su, D. Moira Glerum, and Christopher J. Backhouse, "Rapid simulation of wide-angle scattering from mitochondria in single cells," Opt. Express 16, 12819-12834 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-17-12819


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