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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 17 — Aug. 18, 2008
  • pp: 13315–13322

Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures

Anisha Thayil K. N., Alejandro Muriano, J.-Pablo Salvador, Roger Galve, Maria Pilar Marco, Dobryna Zalvidea, Pablo Loza-Alvarez, Tsvi Katchalski, Eran Grinvald, Asher A. Friesem, and Silvia Soria  »View Author Affiliations


Optics Express, Vol. 16, Issue 17, pp. 13315-13322 (2008)
http://dx.doi.org/10.1364/OE.16.013315


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Abstract

We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm2.

© 2008 Optical Society of America

OCIS Codes
(130.6010) Integrated optics : Sensors
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(230.5750) Optical devices : Resonators

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 17, 2008
Revised Manuscript: August 8, 2008
Manuscript Accepted: August 8, 2008
Published: August 13, 2008

Virtual Issues
Vol. 3, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Anisha Thayil, Alejandro Muriano, J.-Pablo Salvador, Roger Galve, Maria P. Marco, Dobryna Zalvidea, Pablo Loza-Alvarez, Tsvi Katchalski, Eran Grinvald, Asher A. Friesem, and Silvia Soria, "Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures," Opt. Express 16, 13315-13322 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-17-13315


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References

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