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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 19 — Sep. 15, 2008
  • pp: 14723–14730

Delivery of sub-10-fs pulses for nonlinear optical microscopy by polarization-maintaining single mode optical fiber

Adam M. Larson and Alvin T. Yeh  »View Author Affiliations


Optics Express, Vol. 16, Issue 19, pp. 14723-14730 (2008)
http://dx.doi.org/10.1364/OE.16.014723


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Abstract

Broadband, sub-10-fs pulses, can be propagated through polarization-maintaining single mode fiber (PMF) for use in nonlinear optical microscopy (NLOM). We demonstrate delivery of near transform-limited, 1 nJ pulses from a Ti:Al2O3 (75 MHz repetition rate) oscillator via PMF to the NLOM focal plane while maintaining 120 nm of bandwidth. Negative group delay dispersion (GDD) introduced to pre-compensate normal dispersion of the optical fiber and microscope optics ensured linear pulse propagation through the PMF. The minimized time-bandwidth product of the laser pulses at the NLOM focus allowed the nonlinear excitation of multiple fluorophores simultaneously without central wavelength tuning. Polarization sensitive NLOM imaging using second harmonic generation in collagen was demonstrated using PMF delivered pulses. Two-photon excited fluorescence spectra and second harmonic images taken with and without the fiber indicates that the fiber based system is capable of generating optical signals that are within a factor of two to three of our traditional NLOM.

© 2008 Optical Society of America

OCIS Codes
(110.2350) Imaging systems : Fiber optics imaging
(140.7090) Lasers and laser optics : Ultrafast lasers
(190.7110) Nonlinear optics : Ultrafast nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: May 14, 2008
Revised Manuscript: August 19, 2008
Manuscript Accepted: August 21, 2008
Published: September 4, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Adam M. Larson and Alvin T. Yeh, "Delivery of sub-10-fs pulses for nonlinear optical microscopy by polarization-maintaining single mode optical fiber," Opt. Express 16, 14723-14730 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-19-14723


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