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Optics Express

  • Editor: Martijn de Sterke
  • Vol. 16, Iss. 20 — Sep. 29, 2008
  • pp: 16151–16165

Collagen and myosin characterization by orientation field second harmonic microscopy

Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Vincent Fleury, and Yann Le Grand  »View Author Affiliations

Optics Express, Vol. 16, Issue 20, pp. 16151-16165 (2008)

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Collagen and myosin fibrils are endogenous harmonophores that both give rise to Second Harmonic Generation (SHG). By combining four polarization SHG images provided by a scanning microscope, we show that the orientation of the principal axis of the nonlinear susceptibility tensor χ(2) can be determined for each pixel of the image. The ratio ρ=χ33/χ15 of the principal components of χ(2) of collagen and myosin was obtained with the same method, and found within the range 1.6–1.8 and 0.5–0.6 respectively. The orientation of the principal axis of χ(2) is shown to be correlated to the orientation of the fibrils themselves. This provides a straightforward method, which we call Orientation Field-Second Harmonic Microscopy (OF-SHM), to reconstruct orientation fields of fibrils at various scales and resolutions in different biological systems (from muscle sarcomere to the whole embryo).

© 2008 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(160.1435) Materials : Biomaterials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 18, 2008
Revised Manuscript: August 26, 2008
Manuscript Accepted: August 29, 2008
Published: September 26, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Vincent Fleury, and Yann Le Grand, "Collagen and myosin characterization by orientation field second harmonic microscopy," Opt. Express 16, 16151-16165 (2008)

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