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Optics Express

Optics Express

  • Editor: Martijn de Sterke
  • Vol. 16, Iss. 20 — Sep. 29, 2008
  • pp: 16240–16246

Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue, Wonshik Choi, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld  »View Author Affiliations


Optics Express, Vol. 16, Issue 20, pp. 16240-16246 (2008)
http://dx.doi.org/10.1364/OE.16.016240


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Abstract

We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens’ principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

© 2008 Optical Society of America

OCIS Codes
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 1, 2008
Revised Manuscript: September 19, 2008
Manuscript Accepted: September 24, 2008
Published: September 26, 2008

Virtual Issues
Vol. 3, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Niyom Lue, Wonshik Choi, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion," Opt. Express 16, 16240-16246 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-20-16240


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