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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 21 — Oct. 13, 2008
  • pp: 16984–16992

The optical cell rotator

Moritz K. Kreysing, Tobias Kießling, Anatol Fritsch, Christian Dietrich, Jochen R. Guck, and Josef A. Käs  »View Author Affiliations

Optics Express, Vol. 16, Issue 21, pp. 16984-16992 (2008)

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The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy.

© 2008 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(140.3300) Lasers and laser optics : Laser beam shaping
(140.7010) Lasers and laser optics : Laser trapping
(170.1530) Medical optics and biotechnology : Cell analysis
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Trapping and Manipulation

Original Manuscript: September 9, 2008
Revised Manuscript: October 6, 2008
Manuscript Accepted: October 7, 2008
Published: October 9, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Moritz K. Kreysing, Tobias Kießling, Anatol Fritsch, Christian Dietrich, Jochen R. Guck, and Josef A. Käs, "The optical cell rotator," Opt. Express 16, 16984-16992 (2008)

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