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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 16, Iss. 22 — Oct. 27, 2008
  • pp: 18514–18523

Antibody immobilization within glass microstructured fibers: a route to sensitive and selective biosensors

Yinlan Ruan, Tze Cheung Foo, Stephen Warren-Smith, Peter Hoffmann, Roger C. Moore, Heike Ebendorff-Heidepriem, and Tanya M. Monro  »View Author Affiliations


Optics Express, Vol. 16, Issue 22, pp. 18514-18523 (2008)
http://dx.doi.org/10.1364/OE.16.018514


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Abstract

Glass microstructured optical fibers have been rendered biologically active for the first time via the immobilization of antibodies within the holes in the fiber cross-section. This has been done by introducing coating layers to the internal surfaces of soft glass fibers. The detection of proteins that bind to these antibodies has been demonstrated experimentally within this system via the use of fluorescence labeling. The approach combines the sensitivity resulting from the long interaction lengths of filled fibers with the selectivity provided by the use of antibodies.

© 2008 Optical Society of America

OCIS Codes
(060.2370) Fiber optics and optical communications : Fiber optics sensors
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.1030) Spectroscopy : Absorption
(300.2140) Spectroscopy : Emission

ToC Category:
Fiber Optics and Optical Communications

History
Original Manuscript: September 9, 2008
Revised Manuscript: October 21, 2008
Manuscript Accepted: October 21, 2008
Published: October 24, 2008

Virtual Issues
Vol. 3, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Yinlan Ruan, Tze Cheung Foo, Stephen Warren-Smith, Peter Hoffmann, Roger C. Moore, Heike Ebendorff-Heidepriem, and Tanya M. Monro, "Antibody immobilization within glass microstructured fibers: a route to sensitive and selective biosensors," Opt. Express 16, 18514-18523 (2008)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-16-22-18514


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References

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