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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 13 — Jun. 22, 2009
  • pp: 11161–11171

Discrimination of collagen in normal and pathological skin dermis through second-order susceptibility microscopy

Ping-Jung Su, Wei-Liang Chen, Jin-Bon Hong, Tsung-Hsien Li, Ruei-Jr Wu, Chen-Kuan Chou, Shean-Jen Chen, Chieh Hu, Sung-Jan Lin, and Chen-Yuan Dong  »View Author Affiliations

Optics Express, Vol. 17, Issue 13, pp. 11161-11171 (2009)

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Polarization-resolved, second harmonic generation (P-SHG) microscopy at single pixel resolution is utilized for medical diagnosis of pathological skin dermis. In analyzing the large area, pixel by pixel, second-order susceptibility of normal and pathological skin dermis, we found that P-SHG can be used to distinguish normal and dermal pathological conditions of keloid, morphea, and dermal elastolysis. Specifically, we found that the second order susceptibility tensor ratio of d33/d31 for normal skins is 1.27±0.20, while the corresponding values for keloid, morphea, and dermal elastolysis are respectively 1.67±0.29, 1.79±0.30, and 1.75±0.31. We also found that the histograms of the d33/d31 ratio for the pathological skins contain two peak values and are 1.5 times wider than that of the normal case, suggesting that the pathological dermal collagen fibers tend to be more structurally heterogeneous. Our work demonstrates that pixel-resolved, second-order susceptibility microscopy is effective for detecting heterogeneity in spatial distribution of collagen fibers and maybe used for future clinical diagnosis and in vivo studies of collagen pathological conditions.

© 2009 Optical Society of America

OCIS Codes
(170.1870) Medical optics and biotechnology : Dermatology
(190.4160) Nonlinear optics : Multiharmonic generation
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 7, 2009
Revised Manuscript: June 8, 2009
Manuscript Accepted: June 14, 2009
Published: June 19, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Ping-Jung Su, Wei-Liang Chen, Jin-Bon Hong, Tsung-Hsien Li, Ruei-Jr Wu, Chen-Kuan Chou, Shean-Jen Chen, Chieh Hu, Sung-Jan Lin, and Chen-Yuan Dong, "Discrimination of collagen in normal and pathological skin dermis through second-order susceptibility microscopy," Opt. Express 17, 11161-11171 (2009)

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