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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 14 — Jul. 6, 2009
  • pp: 11834–11849

Three-dimensional holographic imaging of living tissue using a highly sensitive photorefractive polymer device

M. Salvador, J. Prauzner, S. Köber, K. Meerholz, J. J. Turek, K. Jeong, and D. D. Nolte  »View Author Affiliations

Optics Express, Vol. 17, Issue 14, pp. 11834-11849 (2009)

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Photorefractive materials are dynamic holographic storage media that are highly sensitive to coherent light fields and relatively insensitive to a uniform light background. This can be exploited to effectively separate ballistic light from multiply-scattered light when imaging through turbid media. We developed a highly sensitive photorefractive polymer composite and incorporated it into a holographic optical coherence imaging system. This approach combines the advantages of coherence-domain imaging with the benefits of holography to form a high-speed wide-field imaging technique. By using coherence-gated holography, image-bearing ballistic light can be captured in real-time without computed tomography. We analyzed the implications of Fourier-domain and image-domain holography on the field of view and image resolution for a transmission recording geometry, and demonstrate holographic depth-resolved imaging of tumor spheroids with 12 µm axial and 10 µm lateral resolution, achieving a data acquisition speed of 8×105 voxels/s.

© 2009 Optical Society of America

OCIS Codes
(100.6890) Image processing : Three-dimensional image processing
(160.5320) Materials : Photorefractive materials
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(090.5694) Holography : Real-time holography

ToC Category:

Original Manuscript: April 30, 2009
Revised Manuscript: May 29, 2009
Manuscript Accepted: June 2, 2009
Published: June 29, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

M. Salvador, J. Prauzner, S. Köber, K. Meerholz, J. J. Turek, K. Jeong, and D. D. Nolte, "Three-dimensional holographic imaging of living tissue using a highly sensitive photorefractive polymer device," Opt. Express 17, 11834-11849 (2009)

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