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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 14 — Jul. 6, 2009
  • pp: 12031–12038

Digital holographic microscopy for quantitative cell dynamic evaluation during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Jun Zhang, Suzanne Genc, Myung K. Kim, Michael W. Berns, and Zhongping Chen  »View Author Affiliations


Optics Express, Vol. 17, Issue 14, pp. 12031-12038 (2009)
http://dx.doi.org/10.1364/OE.17.012031


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Abstract

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system for evaluation of cellular/ sub-cellular dynamic changes during laser micro-dissection. The proposed method takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution. This system will permit quantitative evaluation of the damage and/or the repair of the cell or cell organelles in real time.

© 2009 OSA

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(170.1530) Medical optics and biotechnology : Cell analysis
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Holography

History
Original Manuscript: March 4, 2009
Revised Manuscript: May 18, 2009
Manuscript Accepted: May 19, 2009
Published: July 2, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Lingfeng Yu, Samarendra Mohanty, Jun Zhang, Suzanne Genc, Myung K. Kim, Michael W. Berns, and Zhongping Chen, "Digital holographic microscopy for quantitative cell dynamic evaluation during laser microsurgery," Opt. Express 17, 12031-12038 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-14-12031


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