Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence
                microscopy

doi:10.1364/OE.17.012293

Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy

Liang Gao, Robert T. Kester, and Tomasz S. Tkaczyk »View Author Affiliations

Optics Express, Vol. 17, Issue 15, pp. 12293-12308 (2009)
http://dx.doi.org/10.1364/OE.17.012293

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Abstract

An image slicing spectrometer (ISS) for microscopy applications is presented. Its principle is based on the redirecting of image zones by specially organized thin mirrors within a custom fabricated component termed an image slicer. The demonstrated prototype can simultaneously acquire a 140nm spectral range within its 2D field of view from a single image. The spectral resolution of the system is 5.6nm. The FOV and spatial resolution of the ISS depend on the selected microscope objective and for the results presented is 45 × 45µm² and 0.45µm respectively. This proof-of-concept system can be easily improved in the future for higher (both spectral and spatial) resolution imaging. The system requires no scanning and minimal post data processing. In addition, the reflective nature of the image slicer and use of prisms for spectral dispersion make the system light efficient. Both of the above features are highly valuable for real time fluorescent-spectral imaging in biological and diagnostic applications.

OCIS Codes
(120.4610) Instrumentation, measurement, and metrology : Optical fabrication
(180.2520) Microscopy : Fluorescence microscopy
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Microscopy

History
Original Manuscript: May 6, 2009
Revised Manuscript: June 26, 2009
Manuscript Accepted: June 28, 2009
Published: July 6, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Liang Gao, Robert T. Kester, and Tomasz S. Tkaczyk, "Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy," Opt. Express 17, 12293-12308 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-15-12293

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References

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S. Vivès and E. Prieto, “Original image slicer designed for integral field spectroscopy with the near-infrared spectrograph for the James Webb Space Telescope,” Opt. Eng. 45(9), 093001 (2006). [CrossRef]
F. Henault, R. Bacon, R. Content, B. Lantz, F. Laurent, J. Lemonnier, and S. Morris, “Slicing the universe at affordable cost: the quest for the MUSE image slicer,” Proc. SPIE 5249, 134–145 (2004). [CrossRef]
J. A. Smith, “Basic principles of integral field spectroscopy,” N. Astron. Rev. 50(4-5), 244–251 (2006). [CrossRef]
F. Laurent, F. Henault, E. Renault, R. Bacon, and J. Dubois, “Design of an Integral Field Unit for MUSE, and Results from Prototyping,” Publ. Astron. Soc. Pac. 118(849), 1564–1573 (2006). [CrossRef]
“Mechanisms of 3D intercellular signaling in mammary epithelial cells in response to low dose, low-LET radiation: Implications for the radiation-induced bystander effect,” Biological Sciences Division Research Highlights, Pacific Northwest National Laboratory (2004). http://www.pnl.gov/
W. Preuss and K. Rickens, “Precision machining of integral field units,” N. Astron. Rev. 50(4-5), 332–336 (2006). [CrossRef]
C. M. Dubbeldam, D. J. Robertson, D. A. Ryder, and R. M. Sharples, “Prototyping of Diamond Machined Optics for the KMOS and JWST NIRSpec Integral Field Units,”, ” in Optomechanical Technologies for Astronomy, E. Atad-Ettedgui, J. Antebi, D. Lemke, eds., Proc. SPIE 6273, 62733F (2006).
H. E. Bennett, J. M. Bennett, and E. J. Ashley, “Infrared Reflectance of Evaporated Aluminum Films,” J. Opt. Soc. Am. 52(11), 1245–1250 (1962). [CrossRef]

Ashley, E. J.
Bacon, R.
Barnes, C. A.
Bearman, G.
Belmont, A. S.
Bennett, H. E.
Bennett, J. M.
Bertrand, E.
Brady, D. J.
Buckwald, R. A.
Cabib, D.
Cameron, M.
Chawla, M. K.
Content, R.
Descour, M.
Descour, M. R.
Dubois, J.
Ford, B.
Ford, B. K.
Fraser, S. E.
Garini, Y.
Gehm, M. E.
Genzel, R.
Guzowski, J. F.
Haaland, D. M.
Hamad, S. W.
Haraguchi, T.
Henault, F.
Hiraoka, Y.
Hsu, D. Y.
Janicki, S. M.
John, R.
Jones, H. D. T.
Kosai, Y.
Krabbe, A.
Kroker, H.
Lansford, R.
Lantz, B.
Laurent, F.
Lemonnier, J.
Lin, J. W.
Lipson, S. G.
Lynch, R.
Lynch, R. M.
Malik, Z.
Mathews, S. A.
Matsuoka, H.
McNaughton, B. L.
Melgaard, D. K.
Morris, S.
Murphy, S. M.
Nieman, L. T.
Ntziachristos, V.
Pepperkok, R.
Prasanth, K. V.
Preuss, W.
Prieto, E.
Renault, E.
Rickens, K.
Ried, T.
Rietdorf, J.
Ripoll, J.
Roysam, B.
Sachidanandam, R.
Saito, M.
Salghetti, S. E.
Schulz, T. J.
Shav-Tal, Y.
Shaw, S. Y.
Shimi, T.
Sinclair, M. B.
Singer, R. H.
Smith, J. A.
Spector, D. L.
Sutherland, V. L.
Suto, H.
Tacconi Garman, L. E.
Tacconi-Garman, L. E.
Takeyama, N.
Talmi, A.
Tansey, W. P.
Thatte, N.
Timlin, J. A.
Tsukamoto, T.
Vermaas, W. F. J.
Vivès, S.
Volin, C. E.
Wagadarikar, A.
Wang, L. V.
Weissleder, R.
Weitzel, L.
Willett, R.
Willett, R. M.
Worley, P. F.
Zimmermann, T.

Appl. Opt.

Astron. Astrophys. Suppl. Ser.

L. Weitzel, A. Krabbe, H. Kroker, N. Thatte, L. E. Tacconi-Garman, M. Cameron, R. Genzel, L. E. Tacconi Garman, M. Cameron, and R. Genzel, “3D: The next generation near-infrared imaging spectrometer,” Astron. Astrophys. Suppl. Ser. 119(3), 531–546 (1996). [CrossRef]

Biophys. J.

B. K. Ford, C. E. Volin, S. M. Murphy, R. M. Lynch, and M. R. Descour, “Computed tomography-based spectral imaging for fluorescence microscopy,” Biophys. J. 80(2), 986–993 (2001). [CrossRef] [PubMed]

Cell

S. M. Janicki, T. Tsukamoto, S. E. Salghetti, W. P. Tansey, R. Sachidanandam, K. V. Prasanth, T. Ried, Y. Shav-Tal, E. Bertrand, R. H. Singer, and D. L. Spector, “From silencing to gene expression: real-time analysis in single cells,” Cell 116(5), 683–698 (2004). [CrossRef] [PubMed]

Cell Struct. Funct.

Y. Hiraoka, T. Shimi, and T. Haraguchi, “Multispectral imaging fluorescence microscopy for living cells,” Cell Struct. Funct. 27(5), 367–374 (2002). [CrossRef] [PubMed]

FEBS Lett.

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546(1), 87–92 (2003). [CrossRef] [PubMed]

J. Biomed. Opt.

R. Lansford, G. Bearman, and S. E. Fraser, “Resolution of multiple green fluorescent protein color variants and dyes using two-photon microscopy and imaging spectroscopy,” J. Biomed. Opt. 6(3), 311–318 (2001). [CrossRef] [PubMed]

J. Biotechnol.

H. Matsuoka, Y. Kosai, M. Saito, N. Takeyama, and H. Suto, “Single-cell viability assessment with a novel spectro-imaging system,” J. Biotechnol. 94(3), 299–308 (2002). [CrossRef] [PubMed]

J. Microsc.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, “Fourier transform multipixel spectroscopy for quantitative cytology,” J. Microsc. 182(2), 133–140 (1996). [CrossRef]

J. Neurosci. Methods

V. L. Sutherland, J. A. Timlin, L. T. Nieman, J. F. Guzowski, M. K. Chawla, P. F. Worley, B. Roysam, B. L. McNaughton, M. B. Sinclair, and C. A. Barnes, “Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution,” J. Neurosci. Methods 160(1), 144–148 (2007). [CrossRef]

J. Opt. Soc. Am.

H. E. Bennett, J. M. Bennett, and E. J. Ashley, “Infrared Reflectance of Evaporated Aluminum Films,” J. Opt. Soc. Am. 52(11), 1245–1250 (1962). [CrossRef]

N. Astron. Rev.

J. A. Smith, “Basic principles of integral field spectroscopy,” N. Astron. Rev. 50(4-5), 244–251 (2006). [CrossRef]
W. Preuss and K. Rickens, “Precision machining of integral field units,” N. Astron. Rev. 50(4-5), 332–336 (2006). [CrossRef]

Nat. Biotechnol.

V. Ntziachristos, J. Ripoll, L. V. Wang, and R. Weissleder, “Looking and listening to light: the evolution of whole-body photonic imaging,” Nat. Biotechnol. 23(3), 313–320 (2005). [CrossRef] [PubMed]

Opt. Eng.

S. Vivès and E. Prieto, “Original image slicer designed for integral field spectroscopy with the near-infrared spectrograph for the James Webb Space Telescope,” Opt. Eng. 45(9), 093001 (2006). [CrossRef]

Opt. Express

Proc. Natl. Acad. Sci. U.S.A.

W. F. J. Vermaas, J. A. Timlin, H. D. T. Jones, M. B. Sinclair, L. T. Nieman, S. W. Hamad, D. K. Melgaard, and D. M. Haaland, “In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells,” Proc. Natl. Acad. Sci. U.S.A. 105(10), 4050–4055 (2008). [CrossRef] [PubMed]

Proc. SPIE

F. Henault, R. Bacon, R. Content, B. Lantz, F. Laurent, J. Lemonnier, and S. Morris, “Slicing the universe at affordable cost: the quest for the MUSE image slicer,” Proc. SPIE 5249, 134–145 (2004). [CrossRef]

Publ. Astron. Soc. Pac.

F. Laurent, F. Henault, E. Renault, R. Bacon, and J. Dubois, “Design of an Integral Field Unit for MUSE, and Results from Prototyping,” Publ. Astron. Soc. Pac. 118(849), 1564–1573 (2006). [CrossRef]

Trends Cell Biol.

A. S. Belmont, “Visualizing chromosome dynamics with GFP,” Trends Cell Biol. 11(6), 250–257 (2001). [CrossRef] [PubMed]

Other

ChromoDynamics, Inc., Orlando, FL, “HSi-300 Hyperspectral Imaging System Data Sheet”. http://www.chromodynamics.net/ .
Cambridge Research and Instrumentation, Inc., Cambridge, MA, “VARISPEC Liquid Crystal Tunable Filters Brochure”. http://www.cri-inc.com/
A. Bodkin, A. I. Sheinis, and A. Norton, “Hyperspectral imaging systems,” U. S. Patent 20060072109A1 (2006).
D. M. Haaland, J. A. Timlin, M. B. Sinclair, M. H. V. Benthem, M. J. Matinez, A. D. Aragon, and M. W. Washburne, “Multivariate curve resolution for hyperspectral image analysis: applications to microarray technology,” in Spectral Imaging: Instrumentation, Applications, and Analysis, R. M. Levenson, G. H. Bearman, and A. Mahadevan-Jensen, eds., Proc. SPIE 2959, 55–66 (2003).
C. Zeiss, Germany, “LSM 510 META Product Brochure”. http://www.zeiss.com .
M. A. Rizzo, and D. W. Piston, “Fluorescent Protein Tracking and Detection in Live Cells,” in Live Cell Imaging: A Laboratory Manual, D. Spector and R. Goldman, eds. (Cold Spring Harbor Lab Press, Cold Spring Harbor, NY, 2004).
F. A. Kruse, “Visible-Infrared Sensors and Case Studies,” in Remote Sensing for the Earth Science: Manual of Remote Sensing (3 rd ed.), Renz and N. Andrew, eds. (John Wiley & Sons, NY, 1999).
D. Landgrebe, “Information Extraction Principles and Methods for Multispectral and Hyperspectral Image Data,” in Information Processing for Remote Sensing, C. H. Chen, ed. (World Scientific Publishing Company, River Edge, NY, 1999).
“Mechanisms of 3D intercellular signaling in mammary epithelial cells in response to low dose, low-LET radiation: Implications for the radiation-induced bystander effect,” Biological Sciences Division Research Highlights, Pacific Northwest National Laboratory (2004). http://www.pnl.gov/
C. M. Dubbeldam, D. J. Robertson, D. A. Ryder, and R. M. Sharples, “Prototyping of Diamond Machined Optics for the KMOS and JWST NIRSpec Integral Field Units,”, ” in Optomechanical Technologies for Astronomy, E. Atad-Ettedgui, J. Antebi, D. Lemke, eds., Proc. SPIE 6273, 62733F (2006).

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