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Optics Express

Optics Express

  • Editor: C. Martijn de Sterke
  • Vol. 17, Iss. 18 — Aug. 31, 2009
  • pp: 15585–15591

Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells

Natan T. Shaked, Yizheng Zhu, Matthew T. Rinehart, and Adam Wax  »View Author Affiliations


Optics Express, Vol. 17, Issue 18, pp. 15585-15591 (2009)
http://dx.doi.org/10.1364/OE.17.015585


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Abstract

We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microcopy of live human skin cancer cells.

© 2009 OSA

OCIS Codes
(090.2880) Holography : Holographic interferometry
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 26, 2009
Revised Manuscript: July 30, 2009
Manuscript Accepted: August 11, 2009
Published: August 19, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Natan T. Shaked, Yizheng Zhu, Matthew T. Rinehart, and Adam Wax, "Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells," Opt. Express 17, 15585-15591 (2009)
http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-17-18-15585


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References

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